en
Scientific article
Open access
English

Computational and transcriptional evidence for microRNAs in the honey bee genome

Published inGenomeBiology.com, vol. 8, no. 6, R97
Publication date2007
Abstract

BACKGROUND: Non-coding microRNAs (miRNAs) are key regulators of gene expression in eukaryotes. Insect miRNAs help regulate the levels of proteins involved with development, metabolism, and other life history traits. The recently sequenced honey bee genome provides an opportunity to detect novel miRNAs in both this species and others, and to begin to infer the roles of miRNAs in honey bee development. RESULTS: Three independent computational surveys of the assembled honey bee genome identified a total of 65 non-redundant candidate miRNAs, several of which appear to have previously unrecognized orthologs in the Drosophila genome. A subset of these candidate miRNAs were screened for expression by quantitative RT-PCR and/or genome tiling arrays and most predicted miRNAs were confirmed as being expressed in at least one honey bee tissue. Interestingly, the transcript abundance for several known and novel miRNAs displayed caste or age-related differences in honey bees. Genes in proximity to miRNAs in the bee genome are disproportionately associated with the Gene Ontology terms 'physiological process', 'nucleus' and 'response to stress'. CONCLUSION: Computational approaches successfully identified miRNAs in the honey bee and indicated previously unrecognized miRNAs in the well-studied Drosophila melanogaster genome despite the 280 million year distance between these insects. Differentially transcribed miRNAs are likely to be involved in regulating honey bee development, and arguably in the extreme developmental switch between sterile worker bees and highly fertile queens.

Keywords
  • Animals
  • Bees/ genetics
  • Drosophila melanogaster/genetics
  • Female
  • Gene Expression Regulation, Developmental
  • Genome, Insect
  • Introns
  • MicroRNAs/ genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transcription, Genetic
Citation (ISO format)
WEAVER, D. B. et al. Computational and transcriptional evidence for microRNAs in the honey bee genome. In: GenomeBiology.com, 2007, vol. 8, n° 6, p. R97. doi: 10.1186/gb-2007-8-6-r97
Main files (1)
Article
accessLevelPublic
Identifiers
ISSN of the journal1465-6906
604views
735downloads

Technical informations

Creation07/12/2010 2:36:37 PM
First validation07/12/2010 2:36:37 PM
Update time03/14/2023 3:54:06 PM
Status update03/14/2023 3:54:06 PM
Last indexation02/12/2024 6:59:14 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack