UNIGE document Scientific Article
previous document  unige:9274  next document
add to browser collection
Title

Computational and transcriptional evidence for microRNAs in the honey bee genome

Authors
Weaver, D. B.
Anzola, J. M.
Evans, J. D.
Reid, J. G.
Reese, J. T.
Childs, K. L.
Samanta, M. P.
show hidden authors show all authors [1 - 10]
Published in Genome Biology. 2007, vol. 8, no. 6, p. R97
Abstract BACKGROUND: Non-coding microRNAs (miRNAs) are key regulators of gene expression in eukaryotes. Insect miRNAs help regulate the levels of proteins involved with development, metabolism, and other life history traits. The recently sequenced honey bee genome provides an opportunity to detect novel miRNAs in both this species and others, and to begin to infer the roles of miRNAs in honey bee development. RESULTS: Three independent computational surveys of the assembled honey bee genome identified a total of 65 non-redundant candidate miRNAs, several of which appear to have previously unrecognized orthologs in the Drosophila genome. A subset of these candidate miRNAs were screened for expression by quantitative RT-PCR and/or genome tiling arrays and most predicted miRNAs were confirmed as being expressed in at least one honey bee tissue. Interestingly, the transcript abundance for several known and novel miRNAs displayed caste or age-related differences in honey bees. Genes in proximity to miRNAs in the bee genome are disproportionately associated with the Gene Ontology terms 'physiological process', 'nucleus' and 'response to stress'. CONCLUSION: Computational approaches successfully identified miRNAs in the honey bee and indicated previously unrecognized miRNAs in the well-studied Drosophila melanogaster genome despite the 280 million year distance between these insects. Differentially transcribed miRNAs are likely to be involved in regulating honey bee development, and arguably in the extreme developmental switch between sterile worker bees and highly fertile queens.
Keywords AnimalsBees/ geneticsDrosophila melanogaster/geneticsFemaleGene Expression Regulation, DevelopmentalGenome, InsectIntronsMicroRNAs/ geneticsReverse Transcriptase Polymerase Chain ReactionTranscription, Genetic
Identifiers
PMID: 17543122
Full text
Structures
Research group Génomique Evolutionnaire Computationnelle (830)
Citation
(ISO format)
WEAVER, D. B. et al. Computational and transcriptional evidence for microRNAs in the honey bee genome. In: Genome Biology, 2007, vol. 8, n° 6, p. R97. https://archive-ouverte.unige.ch/unige:9274

228 hits

478 downloads

Update

Deposited on : 2010-07-12

Export document
Format :
Citation style :