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Plasminogen activator inhibitor-1 is induced in migrating endothelial cells

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Published in Journal of Cellular Physiology. 1992, vol. 153, no. 1, p. 129-139
Abstract It has been proposed that a finely tuned protease-anti-protease equilibrium must be maintained during processes of cell migration in order to limit extracellular proteolysis to the close proximity of the cell surface, and thereby to prevent excessive extracellular matrix degradation. We have previously shown that urokinase-type plasminogen activator (u-PA) activity is induced in microvascular endothelial cells migrating from the edges of a wounded monolayer in vitro (Pepper et al., J. Cell Biol., 105:2535-2541, 1987). By Northern analysis, we now demonstrate that plasminogen activator inhibitor 1 (PAI-1) mRNA is increased in multiple-wounded monolayers of bovine microvascular (BME) or aortic (BAE) endothelial cells, with a maximal 7- and 9-fold increase 4 h after wounding, respectively. By in situ hybridization, we demonstrate that the increase in PAI-1 mRNA is localized to cells at the edge of a wounded BME or BAE cell monolayer. The increase in PAI-1 mRNA observed in BME cells is independent of cell division and is inhibited by antibodies to basic fibroblast growth factor (bFGF), suggesting that PAI-1 induction in migrating cells is mediated by the autocrine activity of bFGF. Taken together with our previous observations on the induction of u-PA, these results support the hypothesis that the proteolytic balance in the pericellular environment of migrating cells is regulated through the concomitant production of proteases and protease inhibitors.
Keywords AnimalsBlotting, NorthernCattleCell CountCell Movement/physiologyCells, CulturedEndothelium, Vascular/ cytology/metabolismFibroblast Growth Factor 2/immunology/ physiologyGene Expression Regulation/ physiologyGlycoproteins/geneticsMetalloendopeptidases/geneticsPlasmids/geneticsPlasminogen Inactivators/ metabolismRNA, Messenger/genetics/metabolismTissue Inhibitor of Metalloproteinases
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PMID: 1522127
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PEPPER, Michael Sean et al. Plasminogen activator inhibitor-1 is induced in migrating endothelial cells. In: Journal of Cellular Physiology, 1992, vol. 153, n° 1, p. 129-139. https://archive-ouverte.unige.ch/unige:9243

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Deposited on : 2010-07-12

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