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Scientific article
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Plasminogen activator inhibitor-1 is induced in microvascular endothelial cells by a chondrocyte-derived transforming growth factor-beta

Published inBiochemical and biophysical research communications, vol. 176, no. 2, p. 633-638
Publication date1991
Abstract

We have previously demonstrated that a chondrocyte-derived TGF-beta inhibits spontaneous endothelial sprout formation in an in vitro model of angiogenesis (Pepper et al., J.Cell.Physiol. 146:170-179, 1991). We suggested that the inhibition might be mediated by an antiproteolytic effect. In this paper, we describe the induction of PAI-1 and PAI-1 mRNA in microvascular endothelial cells by chondrocyte conditioned medium. This effect can be significantly reduced by the addition of anti-TGF-beta antibodies to the conditioned medium, and can be mimicked by the addition of exogenous TGF-beta 1. Taken together with our previous observations, these results demonstrate that the inhibition of endothelial sprout formation occurs concomitantly with an increase in the production of PAI-1, a physiological plasminogen activator inhibitor. This suggests that TGF-beta-induced antiproteolysis is responsible for the inhibition of sprout formation.

Keywords
  • Animals
  • Antibodies/immunology
  • Chick Embryo
  • Culture Techniques
  • Endothelium, Vascular/ metabolism
  • Plasminogen Inactivators/immunology/ metabolism
  • Proteins/genetics/immunology/ metabolism
  • RNA, Messenger/metabolism
  • Transforming Growth Factors/genetics/immunology/ metabolism
Citation (ISO format)
PEPPER, Michael Sean et al. Plasminogen activator inhibitor-1 is induced in microvascular endothelial cells by a chondrocyte-derived transforming growth factor-beta. In: Biochemical and biophysical research communications, 1991, vol. 176, n° 2, p. 633–638.
Identifiers
ISSN of the journal0006-291X
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