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Title

Molecular Characzerization of symbiotic Rhizobia and theri legume hosts in situ via Mass Spectrometry
Author
Ziegler, Dominik
Directors
Perret, Xavier
Tonolla, Mauro Amedeo
Defense Thèse de doctorat : Univ. Genève, 2016 - Sc. 5016 - 2016/11/25
Abstract In the field of medical microbiology, matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) secures a rapid identification of clinical isolates. This PhD thesis aimed at implementing MALDI-TOF MS to the characterization of soil bacteria called rhizobia, which form nitrogen-fixing symbioses with plants of the Leguminosae family. Dedicated mass spectral databases and protocols for the rapid characterisation of symbiotic rhizobia were developed. Together these tools allow for a cost-effective high-throughput screening of rhizobia directly from plant tissues instead of cultured bacterial isolates. Taking advantage of the rapidly expending genomic data, we also showed that empirical mass spectra can be replaced by sets of sequence-deduced ribosomal proteins and that plant leghemoglobins are reliable markers for identifying host plants. MALDI-TOF MS has now become a tool of choice to probe the diversity of rhizobia and symbiotic associations that exist in nature, or develop new bio-inoculants for agronomy.
Keywords Rhizobia — Legumes — MALDI-TOF MS — Ribosomal proteins
Identifiers
URN: urn:nbn:ch:unige-923525
Full text
Thesis (24.3 MB) - private document Private access (until 2018-03-06)
Structures
Faculté des sciences / Section de biologie / Département de botanique et biologie végétale
Research group Unité de microbiologie
Citation
(ISO format) 		ZIEGLER, Dominik. Molecular Characzerization of symbiotic Rhizobia and theri legume hosts in situ via Mass Spectrometry. Université de Genève. Thèse, 2016. https://archive-ouverte.unige.ch/unige:92352

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Deposited on : 2017-03-06

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