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Mechanosensitivity of polydiacetylene with a phosphocholine headgroup

Published in Soft Matter. 2017, vol. 13, no. 8, p. 1728-1736
Abstract We demonstrated colorimetric and fluorescence detection of peptide, melittin, with polydiacetylene (PDA) made of 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine (DC(8,9)PC). The PDA used in this work has a phosphocholine headgroup, which mimics peptide-cell membrane interactions better than the conventional PDA assays with carboxyl headgroup. The dose curve (colorimetric response vs melittin concentration) showed a half maximum response at the melittin concentration of 0.1 mg/ml, which is similar to the traditional PDA assays. It suggests that the replacement of the headgroup was achieved without sacrificing the sensitivity. From the dose curve, Hill coefficient was extracted as αHill = 2.1. The value is in agreement with previous melittin studies with standard phospholipids, which reflects the benefit of having a biologically relevant headgroup. In addition, we found an unexpectedly slow spectral change when DC(8,9)PC-PDA was incubated with melittin. The origin of the time-dependent signal was studied by combining UV/VIS spectroscopy, fluorescence spectroscopy and dynamic light scattering.
Keywords PolydiacetyleneMechanochromismDC(8,9)PCMelittinUV/VIS spectroscopyFluorescence spectroscopy
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Other version: http://xlink.rsc.org/?DOI=C6SM02579J
Research group Groupe Sugihara
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ORTUSO, Roberto Diego, CATALDI, Ugo, SUGIHARA, Kaori. Mechanosensitivity of polydiacetylene with a phosphocholine headgroup. In: Soft Matter, 2017, vol. 13, n° 8, p. 1728-1736. doi: 10.1039/C6SM02579J https://archive-ouverte.unige.ch/unige:92341

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Deposited on : 2017-03-06

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