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Title

A clathrin-coated, Golgi-related compartment of the insulin secreting cell accumulates proinsulin in the presence of monensin

Authors
Amherdt, M.
Ravazzola, M.
Perrelet, A.
Published in Cell. 1984, vol. 39, no. 1, p. 39-47
Abstract When the intracellular transit of 3H-labeled (pro)-insulin polypeptides is perturbed by monensin in the pancreatic B-cell, proinsulin conversion is impaired and the radioactive peptides accumulate in a clathrin-coated membrane compartment related to the Golgi apparatus. Clathrin was demonstrated by immunocytochemistry using the postembedding protein A-gold technique. The coated compartment, which is dilated by monensin, comprises Golgi cisternae with condensing secretory material and newly formed secretory granules; under monensin block, the noncoated (storage) secretory granules do not become significantly labeled. These data suggest that an unperturbed passage through a Golgi-related, clathrin-coated membrane compartment which subsequently matures into noncoated secretory granules is needed for the normal processing of (pro)insulin polypeptides.
Keywords AnimalsAutoradiographyClathrin/ analysisFurans/ pharmacologyGolgi Apparatus/drug effects/ metabolism/ultrastructureInsulin/ secretionIslets of Langerhans/drug effects/ metabolism/ultrastructureMicroscopy, ElectronMonensin/ pharmacologyProinsulin/ metabolismRatsTritium/diagnostic use
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PMID: 6386182
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ORCI, Lelio et al. A clathrin-coated, Golgi-related compartment of the insulin secreting cell accumulates proinsulin in the presence of monensin. In: Cell, 1984, vol. 39, n° 1, p. 39-47. https://archive-ouverte.unige.ch/unige:9233

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Deposited on : 2010-07-12

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