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Scientific article
English

A clathrin-coated, Golgi-related compartment of the insulin secreting cell accumulates proinsulin in the presence of monensin

Published inCell, vol. 39, no. 1, p. 39-47
Publication date1984
Abstract

When the intracellular transit of 3H-labeled (pro)-insulin polypeptides is perturbed by monensin in the pancreatic B-cell, proinsulin conversion is impaired and the radioactive peptides accumulate in a clathrin-coated membrane compartment related to the Golgi apparatus. Clathrin was demonstrated by immunocytochemistry using the postembedding protein A-gold technique. The coated compartment, which is dilated by monensin, comprises Golgi cisternae with condensing secretory material and newly formed secretory granules; under monensin block, the noncoated (storage) secretory granules do not become significantly labeled. These data suggest that an unperturbed passage through a Golgi-related, clathrin-coated membrane compartment which subsequently matures into noncoated secretory granules is needed for the normal processing of (pro)insulin polypeptides.

Keywords
  • Animals
  • Autoradiography
  • Clathrin/ analysis
  • Furans/ pharmacology
  • Golgi Apparatus/drug effects/ metabolism/ultrastructure
  • Insulin/ secretion
  • Islets of Langerhans/drug effects/ metabolism/ultrastructure
  • Microscopy, Electron
  • Monensin/ pharmacology
  • Proinsulin/ metabolism
  • Rats
  • Tritium/diagnostic use
Citation (ISO format)
ORCI, Lelio et al. A clathrin-coated, Golgi-related compartment of the insulin secreting cell accumulates proinsulin in the presence of monensin. In: Cell, 1984, vol. 39, n° 1, p. 39–47. doi: 10.1016/0092-8674(84)90189-2
Identifiers
ISSN of the journal0092-8674
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