UNIGE document Scientific Article
previous document  unige:9224  next document
add to browser collection
Title

Plasminogen activator is a mitogen for astrocytes in developing cerebellum

Authors
Moonen, G.
Grau-Wagemans, M. P.
Selak, I.
Lefebvre, P. P.
Rogister, B.
Published in Developmental Brain Research. 1985, vol. 352, no. 1, p. 41-48
Abstract Newborn rat cerebellum microexplants cultured in Minimal Essential Medium with glucose and insulin released plasminogen activator (PA), which was detected in living cultures by a substrate overlay assay. Gel electrophoresis of cerebellum-conditioned medium followed by zymography resolved PA activity in two separate bands of 48,000 and 75,000 daltons apparent mol. wt. Using specific antisera, these bands were shown to be respectively urokinase and tissue-type PA. Cerebellum conditioned medium as well as purified human urokinase induced the proliferation and outgrowth of glial fibrillary acid protein-positive cells from newborn cerebellar microexplants. The effect was suppressed by the serine protease inhibitor phenyl methanesulfonylfluoride. Since PAs are most likely of neuronal origin, we suggest that at least one of these proteases acts as a neuronoglial mitogenic signal during development.
Keywords AnimalsAnimals, NewbornAstrocytes/ drug effectsCerebellum/analysis/ growth & developmentCulture TechniquesMitogens/ analysisPlasminogen Activators/analysis/ pharmacologyRatsRats, Inbred StrainsUrokinase-Type Plasminogen Activator/pharmacology
Identifiers
PMID: 4039964
Full text
This document has no fulltext available yet, but you can contact its author by using the form below.
Structures
Citation
(ISO format)
MOONEN, G. et al. Plasminogen activator is a mitogen for astrocytes in developing cerebellum. In: Developmental Brain Research, 1985, vol. 352, n° 1, p. 41-48. https://archive-ouverte.unige.ch/unige:9224

187 hits

0 download

Update

Deposited on : 2010-07-12

Export document
Format :
Citation style :