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Capillary Electrophoresis-Ultraviolet-Mass Spectrometry (CE-UV-MS) for the Simultaneous Determination and Quantification of Insulin Formulations

Published inCapillary Electrophoresis of Proteins and Peptides - Methods and Protocols, Editors Nguyet Thuy Tran and Myriam Taverna
PublisherFrance : Humana Press
Publication date2016
Abstract

This chapter describes a CE-UV-MS method for the identification and quantification of insulin in pharmaceutical formulations in a single run. The CE conditions are optimized to avoid the adsorption of the protein onto the capillary wall. Particular attention is paid regarding the choice of the internal standard. A strategy based on multiple injections is applied to correct both ionization and injection variabilities. The methodoligy is validated according to internal guidelines and the obtained accuracy profile demonstrates the ability of the CE-UV-MS method to quantify insulin in pharmaceutical formulations within a 15% acceptance range. This strategy can be implemented in the field of quality control, as well as in the detection of counterfeits.

Keywords
  • Capillary electrophoresis
  • Identification
  • Insulin
  • Intact protein
  • Multiple injections
  • Qualitcontrol
  • Quantification
  • Mass spectrometry
Citation (ISO format)
SCHAPPLER, Julie, RUDAZ, Serge. Capillary Electrophoresis-Ultraviolet-Mass Spectrometry (CE-UV-MS) for the Simultaneous Determination and Quantification of Insulin Formulations. In: Capillary Electrophoresis of Proteins and Peptides - Methods and Protocols. France : Humana Press, 2016.
Main files (1)
Book chapter (Published version)
accessLevelPublic
Identifiers
  • PID : unige:92042
ISBN978-1-4939-4012-7
589views
241downloads

Technical informations

Creation02/22/2017 9:39:00 AM
First validation02/22/2017 9:39:00 AM
Update time03/15/2023 1:23:55 AM
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