UNIGE document Doctoral Thesis
previous document  unige:91833  next document
add to browser collection
Title

Regulation of dynamin-mediated membrane fission by the N-BAR protein endophilin

Author
Director
Defense Thèse de doctorat : Univ. Genève, 2016 - Sc. 5031 - 2016/12/16
Abstract Eukaryotic cells are composed of different compartments with defined biological functions. Transport of molecules from one compartment to another, as well as from and to the plasma membrane, occurs via membrane vesicles. Clathrin-mediated endocytosis is the most studied pathway for the formation of such a vesicle. The GTPase dynamin mediates the final membrane fission step in this process. This thesis describes the experimental work to clarify how a specific class of crescent-shaped proteins called N-BAR proteins regulate dynamin-mediated membrane fission. Endophilin, one of the N-BAR proteins, was taken as an example. The major experimental technique is the nanotube pulling assay, an in-vitro essay using purified proteins. Other chapters of this thesis explore curvature-inducing properties of the clathrin adapter AP180 and the curvature-dependent activity of yeast Vps34 complex I. Additionally, this thesis analyses structural consequences of mutations in dynamin and another N-BAR protein, BIN1/amphiphysin 2, which cause the hereditary muscle disease CNM (centronuclear myopathy).
Keywords TransportCell biologyDynaminDynamineEndophilinEndophilineMembrane fissionFission membranaireN-BARBARMembraneIn vitroClathrinClathrineAP180AmphiphysinAmphiphysineBIN1CNMCentronuclear myopathyMCNMyopathie centronucléaireMutationsGenetic diseaseVps34Nanotube pulling assayCMEClathrin-mediated endocytosisEndocytose médiée par la clathrine
Identifiers
URN: urn:nbn:ch:unige-918330
Full text
Thesis (45.3 MB) - document accessible for UNIGE members only Limited access to UNIGE (until 2018-01-31)
Structures
Research group Groupe Roux
Projects 31003A-149975
RGY0076/6/2009
Citation
(ISO format)
HOHENDAHL, Annika. Regulation of dynamin-mediated membrane fission by the N-BAR protein endophilin. Université de Genève. Thèse, 2016. https://archive-ouverte.unige.ch/unige:91833

96 hits

2 downloads

Update

Deposited on : 2017-02-13

Export document
Format :
Citation style :