UNIGE document Scientific Article
previous document  unige:91618  next document
add to browser collection
Title

Cell Cycle Constraints and Environmental Control of Local DNA Hypomethylation in α-Proteobacteria

Authors
Russo, Giancarlo
Patrignani, Andrea
Schlapbach, Ralph
show hidden authors show all authors [1 - 9]
Published in PLOS Genetics. 2016, vol. 12, no. 12, p. e1006499
Abstract Heritable DNA methylation imprints are ubiquitous and underlie genetic variability from bacteria to humans. In microbial genomes, DNA methylation has been implicated in gene transcription, DNA replication and repair, nucleoid segregation, transposition and virulence of pathogenic strains. Despite the importance of local (hypo)methylation at specific loci, how and when these patterns are established during the cell cycle remains poorly characterized. Taking advantage of the small genomes and the synchronizability of α-proteobacteria, we discovered that conserved determinants of the cell cycle transcriptional circuitry establish specific hypomethylation patterns in the cell cycle model system Caulobacter crescentus. We used genome-wide methyl-N6-adenine (m6A-) analyses by restriction-enzyme-cleavage sequencing (REC-Seq) and single-molecule real-time (SMRT) sequencing to show that MucR, a transcriptional regulator that represses virulence and cell cycle genes in S-phase but no longer in G1-phase, occludes 5'-GANTC-3' sequence motifs that are methylated by the DNA adenine methyltransferase CcrM. Constitutive expression of CcrM or heterologous methylases in at least two different α-proteobacteria homogenizes m6A patterns even when MucR is present and affects promoter activity. Environmental stress (phosphate limitation) can override and reconfigure local hypomethylation patterns imposed by the cell cycle circuitry that dictate when and where local hypomethylation is instated.
Identifiers
PMID: 27997543
Full text
Article (Published version) (4.7 MB) - public document Free access
Structures
Research group Régulation du cycle cellulaire dans C.crescentus, une bactérie asymétrique (895)
Citation
(ISO format)
ARDISSONE, Silvia et al. Cell Cycle Constraints and Environmental Control of Local DNA Hypomethylation in α-Proteobacteria. In: PLOS Genetics, 2016, vol. 12, n° 12, p. e1006499. https://archive-ouverte.unige.ch/unige:91618

92 hits

9 downloads

Update

Deposited on : 2017-02-03

Export document
Format :
Citation style :