en
Scientific article
English

Antagonist effect of RU 486 on transcription of glucocorticoid-regulated genes

Published inExperimental cell research, vol. 173, no. 2, p. 425-430
Publication date1987
Abstract

The effect of RU 486, a synthetic steroid that is a powerful antagonist of glucocorticoid hormones, was tested on the transcription of several glucocorticoid-regulated genes in different cell types: inflammatory murine macrophages and two human mammary gland-derived cell lines, MDA-MB-231 and HBL-100. The transcription of genes which are positively regulated by glucocorticoids (e.g., tissue-type plasminogen activator and c-myc in mammary cells, c-fos in macrophages) and that of genes which are negatively regulated by these agents (e.g., urokinase-type plasminogen activator in all three cell types, TNF-a and IL-1 in macrophages) was explored. RU 486 almost completely prevented the effects of dexamethasone on the transcription of these various genes. When added alone, RU 486 had essentially no agonist activity.

Keywords
  • Animals
  • Cell Line, Transformed
  • Dexamethasone/ antagonists & inhibitors/pharmacology
  • Estrenes/ pharmacology
  • Humans
  • Interleukin-1/genetics
  • Macrophages/enzymology
  • Mice
  • Mifepristone
  • Plasminogen Activators/ genetics/metabolism
  • RNA, Messenger/biosynthesis
  • Tissue Plasminogen Activator/ genetics/metabolism
  • Transcription, Genetic/ drug effects
  • Tumor Cells, Cultured
  • Tumor Necrosis Factor-alpha/genetics
  • Urokinase-Type Plasminogen Activator/ genetics/metabolism
Citation (ISO format)
BUSSO, N. et al. Antagonist effect of RU 486 on transcription of glucocorticoid-regulated genes. In: Experimental cell research, 1987, vol. 173, n° 2, p. 425–430.
Identifiers
ISSN of the journal0014-4827
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