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Title

Détection ultrastructurale de l'hétérogénéité du glycocalyx dans les tubes contournés et droits proximaux du rein de rat par la technique de la lectine couplée à l'or

Authors
Brown, D.
Kunz, A.
Published in Comptes Rendus des Séances de l'Académie des Sciences. III: Sciences de la Vie. 1983, vol. 297, no. 10, p. 501-506
Abstract A new histochemical technique in which lectin-gold complexes are applied directly to thin and semi-thin sections of tissue was used to determine the distribution of Helix pomatia lectin binding sites in the proximal tubule of Rat kidney. Whereas a strong labeling was seen on the brush border and baso-lateral plasma membranes of convoluted tubules, the plasma membrane of cells from the medullary straight segment was unlabeled. Some profiles at the frontier between cortex and medulla had a mosaic of positive and negative cells. These results show, therefore, considerable segmental variation in the glycocalyx composition of Rat proximal tubules.
Keywords AnimalsGlycoproteins/ analysisGoldHistocytochemistryKidney Tubules, Proximal/analysis/ ultrastructureLectinsMicroscopy, ElectronPolysaccharides/ analysisRatsRats, Inbred StrainsReceptors, Mitogen/analysis
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PMID: 6419990
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BROWN, D. et al. Détection ultrastructurale de l'hétérogénéité du glycocalyx dans les tubes contournés et droits proximaux du rein de rat par la technique de la lectine couplée à l'or. In: Comptes Rendus des Séances de l'Académie des Sciences. III: Sciences de la Vie, 1983, vol. 297, n° 10, p. 501-506. https://archive-ouverte.unige.ch/unige:9147

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