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Détection ultrastructurale de l'hétérogénéité du glycocalyx dans les tubes contournés et droits proximaux du rein de rat par la technique de la lectine couplée à l'or

Publication date1983
Abstract

A new histochemical technique in which lectin-gold complexes are applied directly to thin and semi-thin sections of tissue was used to determine the distribution of Helix pomatia lectin binding sites in the proximal tubule of Rat kidney. Whereas a strong labeling was seen on the brush border and baso-lateral plasma membranes of convoluted tubules, the plasma membrane of cells from the medullary straight segment was unlabeled. Some profiles at the frontier between cortex and medulla had a mosaic of positive and negative cells. These results show, therefore, considerable segmental variation in the glycocalyx composition of Rat proximal tubules.

Keywords
  • Animals
  • Glycoproteins/ analysis
  • Gold
  • Histocytochemistry
  • Kidney Tubules, Proximal/analysis/ ultrastructure
  • Lectins
  • Microscopy, Electron
  • Polysaccharides/ analysis
  • Rats
  • Rats, Inbred Strains
  • Receptors, Mitogen/analysis
Citation (ISO format)
BROWN, D. et al. Détection ultrastructurale de l’hétérogénéité du glycocalyx dans les tubes contournés et droits proximaux du rein de rat par la technique de la lectine couplée à l’or. In: Comptes rendus des séances de l’Académie des sciences. Série 3, Sciences de la vie, 1983, vol. 297, n° 10, p. 501–506.
Identifiers
ISSN of the journal0249-6313
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