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Scientific article
English

The 21q22.1 STS marker, VN02 (EST00541 cDNA), is part of the 3' sequence of the human Na+/myo-inositol cotransporter (SLC5A3) gene

Published inCytogenetics and cell genetics, vol. 73, no. 1-2, p. 77-78
Publication date1996
Abstract

The human osmoregulatory Na+/myo-inositol cotransporter gene (SLC5A3) was recently cloned and localized to the region of 21q22. Fine mapping of this gene was accomplished by identifying YAC clones that contain SLC5A3 and utilizing known STS markers for 21q22.1 and 21q22.2 sub-bands that map to the positive YAC clones. Two bacteriophage P1 clones containing the SLC5A3 gene gave a positive PCR product when screened with the 21q22.1 marker VN02, an expressed sequence tag (EST00541). Through DNA sequence analysis, it was determined that this STS marker if part of the 3' untranslated region of the SLC5A3 gene.

Keywords
  • Bacteriophages/genetics
  • Base Sequence
  • Carrier Proteins/ genetics
  • Chromosomes, Human, Pair 21/ genetics
  • DNA Primers
  • DNA, Complementary/genetics
  • Gene Expression
  • Genes
  • Genetic Markers
  • Heat-Shock Proteins/ genetics
  • Humans
  • Membrane Proteins
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Symporters
Citation (ISO format)
BERRY, G. T. et al. The 21q22.1 STS marker, VN02 (EST00541 cDNA), is part of the 3′ sequence of the human Na+/myo-inositol cotransporter (SLC5A3) gene. In: Cytogenetics and cell genetics, 1996, vol. 73, n° 1-2, p. 77–78. doi: 10.1159/000134311
Identifiers
ISSN of the journal0301-0171
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