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Scientific article
English

The receptor for urokinase-type plasminogen activator is expressed during mouse spermatogenesis

Published inFEBS letters, vol. 413, no. 1, p. 11-15
Publication date1997
Abstract

Urokinase-type plasminogen activator (uPA) binds to a plasma membrane receptor (uPAR) that localizes plasmin generation to the cell environment. Mouse spermatozoa have surface-bound uPA, which appears to be acquired from genital tract secretions at ejaculation. We determined the presence of uPAR mRNA in spermatogenic cells and their uPA-binding activity. Northern blot and in situ hybridization demonstrated the presence of uPAR mRNA in germ cells. Binding of uPA, but not of a mutant enzyme lacking the receptor-binding domain, indicated the presence of uPAR on spermatids and spermatozoa. The uPAR and/or receptor-bound uPA may be involved in spermatogenesis, spermatozoa maturation or fertilization.

Keywords
  • Animals
  • Blotting, Northern
  • Germ Cells/metabolism
  • In Situ Hybridization
  • Male
  • Mice
  • Mice, Knockout
  • Plasminogen Activators/deficiency/ metabolism
  • RNA, Messenger/metabolism
  • Receptors, Cell Surface/deficiency/ metabolism
  • Receptors, Urokinase Plasminogen Activator
  • Spermatids/metabolism
  • Spermatogenesis
  • Spermatozoa/metabolism
Citation (ISO format)
ZHOU, H., VASSALLI, Jean-Dominique. The receptor for urokinase-type plasminogen activator is expressed during mouse spermatogenesis. In: FEBS letters, 1997, vol. 413, n° 1, p. 11–15. doi: 10.1016/s0014-5793(97)00861-2
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ISSN of the journal0014-5793
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