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Cingulin interacts with F-actin in vitro

D'atri, Fabio
Published in FEBS Letters. 2001, vol. 507, no. 1, p. 21-24
Abstract Cingulin, a M r 140–160 kDa protein of the cytoplasmic plaque of epithelial tight junctions (TJ), interacts in vitro with TJ proteins and myosin. Here we investigated cingulin interaction with actin, using His-tagged, full-length Xenopus laevis cingulin expressed in insect cells, and glutathione S-transferase (GST) fusion proteins of fragments of cingulin expressed in bacteria. Purified full-length cingulin co-pelleted with F-actin after high speed centrifugation, and promoted the sedimentation of F-actin under low speed centrifugation, suggesting that cingulin is an actin-cross-linking protein. The actin interaction of GST fusion proteins containing fragments of Xenopus cingulin suggested that the F-actin binding site is between residues 101 and 294.
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D'ATRI, Fabio, CITI, Sandra. Cingulin interacts with F-actin in vitro. In: FEBS Letters, 2001, vol. 507, n° 1, p. 21-24. https://archive-ouverte.unige.ch/unige:90951

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