fr
Article scientifique
Anglais

A high-resolution physical map of human chromosome 21p using yeast artificial chromosomes

Publié dansGenome research, vol. 9, no. 11, p. 1059-1073
Date de publication1999
Résumé

The short arm of human chromosome 21 (21p) contains many different types of repetitive sequences and is highly homologous to the short arms of other acrocentric chromosomes. Owing to its repetitive nature and the lack of chromosome 21p-specific molecular markers, most physical maps of chromosome 21 exclude this region. We constructed a physical map of chromosome 21p using sequence tagged site (STS) content mapping of yeast artificial chromosomes (YACs). To this end, 39 STSs located on the short arm or near the centromere of chromosome 21 were constructed, including four polymorphic simple tandem repeats (STRs) and two expressed sequence tags (ESTs). Thirty YACs were selected from the St. Louis YAC library, the chromosome 21-enriched ICRF YAC library, and the CEPH YAC and megaYAC libraries. These were assembled in a YAC contig map ranging from the centromere to the rDNA gene cluster at 21p12. The total size of the region covered by YACs is estimated between 2.9 and 5 Mb. The integrity of the YAC contig was confirmed by restriction enzyme fingerprinting and fluorescence in situ hybridization (FISH). One gap with an estimated size of 400 kb remained near the telomeric end of the contig. This YAC contig map of the short arm of human chromosome 21 constitutes a basic framework for further structural and functional studies of chromosome 21p.

Mots-clés
  • Chromosomes, Artificial, Yeast
  • Chromosomes, Human, Pair 21/ genetics
  • Contig Mapping/methods
  • Expressed Sequence Tags
  • Female
  • Humans
  • In Situ Hybridization, Fluorescence
  • Male
  • Metaphase
  • Pedigree
  • Physical Chromosome Mapping/methods
  • Sequence Tagged Sites
Citation (format ISO)
WANG, S. Y. et al. A high-resolution physical map of human chromosome 21p using yeast artificial chromosomes. In: Genome research, 1999, vol. 9, n° 11, p. 1059–1073. doi: 10.1101/gr.9.11.1059
Fichiers principaux (1)
Article
accessLevelRestricted
Identifiants
ISSN du journal1088-9051
550vues
0téléchargements

Informations techniques

Création12.07.2010 12:01:57
Première validation12.07.2010 12:01:57
Heure de mise à jour14.03.2023 15:53:15
Changement de statut14.03.2023 15:53:14
Dernière indexation12.02.2024 18:58:29
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack