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Scientific article
English

Regulated polyadenylation controls mRNA translation during meiotic maturation of mouse oocytes

Published inGenes & development, vol. 3, no. 12B, p. 2163-2171
Publication date1989
Abstract

The translational activation of dormant tissue-type plasminogen activator mRNA during meiotic maturation of mouse oocytes is accompanied by elongation of its 3'-poly(A) tract. Injected RNA fragments that correspond to part of the 3'-untranslated region (3'UTR) of this mRNA are also subject to regulated polyadenylation. Chimeric mRNAs containing part of this 3'UTR are polyadenylated and translated following resumption of meiosis. Polyadenylation and translation of chimeric mRNAs require both specific sequences in the 3'UTR and the canonical 3'-processing signal AAUAAA. Injection of 3'-blocked mRNAs and in vitro polyadenylated mRNAs shows that the presence of a long poly(A) tract is necessary and sufficient for translation. These results establish a role for regulated polyadenylation in the post-transcriptional control of gene expression.

Keywords
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Chimera/genetics
  • Meiosis
  • Mice
  • Molecular Sequence Data
  • Oocytes/cytology/ metabolism
  • Poly A/ metabolism
  • Protein Biosynthesis
  • RNA/genetics
  • RNA, Messenger/ genetics/metabolism
  • Tissue Plasminogen Activator/ genetics
Citation (ISO format)
VASSALLI, Jean-Dominique et al. Regulated polyadenylation controls mRNA translation during meiotic maturation of mouse oocytes. In: Genes & development, 1989, vol. 3, n° 12B, p. 2163–2171. doi: 10.1101/gad.3.12b.2163
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ISSN of the journal0890-9369
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