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Regulated polyadenylation controls mRNA translation during meiotic maturation of mouse oocytes

Gubler, P.
Vassalli, A.
O'Connell, M. L.
Parton, L. A.
Rickles, R. J.
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Published in Genes and Development. 1989, vol. 3, no. 12B, p. 2163-2171
Abstract The translational activation of dormant tissue-type plasminogen activator mRNA during meiotic maturation of mouse oocytes is accompanied by elongation of its 3'-poly(A) tract. Injected RNA fragments that correspond to part of the 3'-untranslated region (3'UTR) of this mRNA are also subject to regulated polyadenylation. Chimeric mRNAs containing part of this 3'UTR are polyadenylated and translated following resumption of meiosis. Polyadenylation and translation of chimeric mRNAs require both specific sequences in the 3'UTR and the canonical 3'-processing signal AAUAAA. Injection of 3'-blocked mRNAs and in vitro polyadenylated mRNAs shows that the presence of a long poly(A) tract is necessary and sufficient for translation. These results establish a role for regulated polyadenylation in the post-transcriptional control of gene expression.
Keywords AnimalsBase SequenceBlotting, NorthernChimera/geneticsMeiosisMiceMolecular Sequence DataOocytes/cytology/ metabolismPoly A/ metabolismProtein BiosynthesisRNA/geneticsRNA, Messenger/ genetics/metabolismTissue Plasminogen Activator/ genetics
PMID: 2483395
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Other version: http://genesdev.cshlp.org/content/3/12b/2163.full.pdf
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VASSALLI, Jean-Dominique et al. Regulated polyadenylation controls mRNA translation during meiotic maturation of mouse oocytes. In: Genes and Development, 1989, vol. 3, n° 12B, p. 2163-2171. doi: 10.1101/gad.3.12b.2163 https://archive-ouverte.unige.ch/unige:9069

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Deposited on : 2010-07-12

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