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Title

Mapping of tetraspanin-enriched microdomains that can function as gateways for HIV-1

Authors
Nydegger, Sascha
Khurana, Sandhya
Krementsov, Dimitry N
Thali, Markus
Published in The Journal of Cell Biology. 2006, vol. 173, no. 5, p. 795-807
Abstract Specific spatial arrangements of proteins and lipids are central to the coordination of many biological processes. Tetraspanins have been proposed to laterally organize cellular membranes via specific associations with each other and with distinct integrins. Here, we reveal the presence of tetraspanin-enriched microdomains (TEMs) containing the tetraspanins CD9, CD63, CD81, and CD82 at the plasma membrane. Fluorescence and immunoelectron microscopic analyses document that the surface of HeLa cells is covered by several hundred TEMs, each extending over a few hundred nanometers and containing predominantly two or more tetraspanins. Further, we reveal that the human immunodeficiency virus type 1 (HIV-1) Gag protein, which directs viral assembly and release, accumulates at surface TEMs together with the HIV-1 envelope glycoprotein. TSG101 and VPS28, components of the mammalian ESCRT1 (endosomal sorting complex required for transport), which is part of the cellular extravesiculation machinery critical for HIV-1 budding, are also recruited to cell surface TEMs upon virus expression, suggesting that HIV-1 egress can be gated through these newly mapped microdomains.
Keywords AntigensCD/chemistry/metabolismAntigensCD63AntigensCD81AntigensCD82/chemistry/metabolismAntigensCD9Cell Membrane/metabolism/virologyGene ProductsEnv/metabolismGene ProductsGag/metabolismHIV-1/metabolismHeLa CellsHumansJurkat CellsMembrane Glycoproteins/chemistry/metabolismMembrane Microdomains/metabolism/virologyMembrane Proteins/metabolismPlatelet Membrane Glycoproteins/chemistry/metabolismSurface PropertiesT-Lymphocytes/metabolism/virology
Identifiers
PMID: 16735575
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Article (Published version) (4 MB) - document accessible for UNIGE members only Limited access to UNIGE
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Research group Obésité et syndrome métabolique (803)
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NYDEGGER, Sascha et al. Mapping of tetraspanin-enriched microdomains that can function as gateways for HIV-1. In: The Journal of Cell Biology, 2006, vol. 173, n° 5, p. 795-807. https://archive-ouverte.unige.ch/unige:90669

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Deposited on : 2016-12-21

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