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HIV-1 egress is gated through late endosomal membranes

Nydegger, Sascha
Derdowski, Aaron
Spearman, Paul
Thali, Markus
Published in Traffic. 2003, vol. 4, no. 12, p. 902-10
Abstract HIV-1 buds from the surface of activated T lymphocytes. In macrophages, however, newly formed HIV-1 particles amass in the lumen of an intracellular compartment. Here, we demonstrate by live-cell imaging techniques, by immunocytochemistry and by immuno-electron microscopy that HIV-1 structural proteins, particularly the internal structural protein Gag, accumulate at membranes of the late endocytic compartment in a variety of cell types and not just in monocyte/macrophage-derived cells. Recent biochemical and genetic studies have implicated components of the mammalian vacuolar protein sorting pathway in retroviral budding. Together with those observations, our study suggests that HIV-1 morphogenesis is thoroughly rooted in the endosomal system.
Keywords Cell Membrane/metabolismEndosomes/metabolism/virologyGene ProductsGag/metabolismGreen Fluorescent ProteinsHIV-1/metabolismHeLa CellsHumansImmunohistochemistryK562 CellsLuminescent Proteins/metabolismMicroscopyElectronMicroscopyFluorescenceMicroscopyImmunoelectronPlasmids/metabolismProtein StructureTertiaryRecombinant Fusion Proteins/metabolismRetroviridae/geneticsTransfectionVirus Replication
PMID: 14617353
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Research group Obésité et syndrome métabolique (803)
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NYDEGGER, Sascha et al. HIV-1 egress is gated through late endosomal membranes. In: Traffic, 2003, vol. 4, n° 12, p. 902-10. https://archive-ouverte.unige.ch/unige:90662

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Deposited on : 2016-12-21

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