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Title

The HIV-1 Nef protein acts as a connector with sorting pathways in the Golgi and at the plasma membrane

Authors
Mangasarian, A
Aiken, C
Chin, D
Carpentier, J L
Trono, D
Published in Immunity. 1997, vol. 6, no. 1, p. 67-77
Abstract The HIV Nef protein down-regulates the cell surface expression of CD4 and of MHC I at least in part through accelerated endocytosis. To investigate further the mechanism of this effect, we created chimeric integral membrane proteins comprising the extracellular and transmembrane regions of CD4 or CD8 and Nef as the cytoplasmic domain. These fusion molecules could down-modulate CD4 in trans in a dileucine-dependent manner. Furthermore, in spite of lacking receptor-derived internalization signals, the Nef-containing chimeras underwent both Golgi retention and rapid endocytosis via clathrin-coated pits. Taken together, these data suggest that Nef down-regulates CD4 and probably MHC I by physically connecting these receptors with sorting pathways in the Golgi and at the plasma membrane.
Keywords AntigensCD4/metabolismAntigensCD8/metabolismCell CompartmentationCell Membrane/metabolismCoated PitsCell-Membrane/metabolismCytoplasm/chemistry/metabolismDown-RegulationEndocytosisGene ProductsNef/metabolismGolgi Apparatus/metabolismHIV-1/metabolismHumansMicroscopyConfocalRecombinant Fusion Proteins/metabolismNef Gene ProductsHuman Immunodeficiency Virus
Identifiers
PMID: 9052838
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Structures
Research group Obésité et syndrome métabolique (803)
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MANGASARIAN, A et al. The HIV-1 Nef protein acts as a connector with sorting pathways in the Golgi and at the plasma membrane. In: Immunity, 1997, vol. 6, n° 1, p. 67-77. https://archive-ouverte.unige.ch/unige:90654

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Deposited on : 2016-12-21

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