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The TPTE gene family: cellular expression, subcellular localization and alternative splicing

Guillou, Louis
Lamon, Christine
Published in Gene. 2003, vol. 323, p. 189-199
Abstract The human TPTE (Transmembrane Phosphatase with TEnsin homology) gene family encodes a PTEN-related tyrosine phosphatase with four potential transmembrane domains. Chromosomal mapping revealed multiple copies of the TPTE gene on chromosomes 13, 15, 21, 22 and Y. Human chromosomes 13 and 21 copies encode two functional proteins, TPIP (TPTE and PTEN homologous Inositol lipid Phosphatase) and TPTE, respectively, whereas only one copy of the gene exists in the mouse genome. In the present study, we show that TPTE and TPIP proteins are expressed in secondary spermatocytes and/or prespermatids. In addition, we report the existence of several novel alternatively spliced isoforms of these two proteins with variable number of transmembrane domains. The latter has no influence on the subcellular localization of these different peptides as shown by co-immunofluorescence experiments. Finally, we identify another expressed TPTE copy, mapping to human chromosome 22, whose transcription appears to be under the control of the LTR of human endogenous retrovirus RTVL-H3.
Keywords Alternative SplicingAmino Acid SequenceAnimalsBase SequenceCOS CellsCell LineCercopithecus aethiopsChromosomes, Human, Pair 22/geneticsDNA, Complementary/chemistry/geneticsFemaleGene Expression Regulation, EnzymologicGreen Fluorescent ProteinsHela CellsHumansImmunohistochemistryLuminescent Proteins/genetics/metabolismMaleMembrane Proteins/ genetics/metabolismMicroscopy, FluorescenceMolecular Sequence DataMutationPTEN PhosphohydrolasePhosphoric Monoester Hydrolases/genetics/metabolismProtein Isoforms/genetics/metabolismProtein Tyrosine Phosphatases/ genetics/metabolismPseudogenes/geneticsRecombinant Fusion Proteins/genetics/metabolismSequence Analysis, DNASpermatids/enzymologySpermatocytes/enzymologyTesticular Neoplasms/enzymology/genetics/pathologyTestis/enzymology
PMID: 14659893
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TAPPAREL, Caroline et al. The TPTE gene family: cellular expression, subcellular localization and alternative splicing. In: Gene, 2003, vol. 323, p. 189-199. doi: 10.1016/j.gene.2003.09.038 https://archive-ouverte.unige.ch/unige:9050

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Deposited on : 2010-07-12

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