

Other version: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC317088/pdf/x4.pdf
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Masking, unmasking, and regulated polyadenylation cooperate in the translational control of a dormant mRNA in mouse oocytes |
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Published in | Genes and Development. 1998, vol. 12, no. 16, p. 2535-2548 | |
Abstract | The mechanisms responsible for translational silencing of certain mRNAs in growing oocytes, and for their awakening during meiotic maturation, are not completely elucidated. We show that binding of a approximately 80-kD protein to a UA-rich element in the 3' UTR of tissue-type plasminogen activator mRNA, a mouse oocyte mRNA that is translated during meiotic maturation, silences the mRNA in primary oocytes. Translation can be triggered by injecting a competitor transcript that displaces this silencing factor, without elongation of a pre-existing short poly(A) tail, the presence of which is mandatory. During meiotic maturation, cytoplasmic polyadenylation is necessary to maintain a poly(A) tail, but the determining event for translational activation appears to be the modification or displacement of the silencing factor. | |
Keywords | Animals — Binding, Competitive — Cells, Cultured — Cytoplasm/metabolism — Meiosis/genetics — Mice — Oocytes/ physiology — Oogenesis/genetics — Protein Biosynthesis — RNA, Messenger/ physiology — Tissue Plasminogen Activator/genetics/metabolism | |
Identifiers | PMID: 9716406 | |
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![]() ![]() Other version: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC317088/pdf/x4.pdf |
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Citation (ISO format) | STUTZ, A. et al. Masking, unmasking, and regulated polyadenylation cooperate in the translational control of a dormant mRNA in mouse oocytes. In: Genes & development, 1998, vol. 12, n° 16, p. 2535-2548. doi: 10.1101/gad.12.16.2535 https://archive-ouverte.unige.ch/unige:9046 |