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Title

Masking, unmasking, and regulated polyadenylation cooperate in the translational control of a dormant mRNA in mouse oocytes

Authors
Stutz, A.
Gubler, P.
Volkel, V.
Flandin, P.
Published in Genes and Development. 1998, vol. 12, no. 16, p. 2535-2548
Abstract The mechanisms responsible for translational silencing of certain mRNAs in growing oocytes, and for their awakening during meiotic maturation, are not completely elucidated. We show that binding of a approximately 80-kD protein to a UA-rich element in the 3' UTR of tissue-type plasminogen activator mRNA, a mouse oocyte mRNA that is translated during meiotic maturation, silences the mRNA in primary oocytes. Translation can be triggered by injecting a competitor transcript that displaces this silencing factor, without elongation of a pre-existing short poly(A) tail, the presence of which is mandatory. During meiotic maturation, cytoplasmic polyadenylation is necessary to maintain a poly(A) tail, but the determining event for translational activation appears to be the modification or displacement of the silencing factor.
Keywords AnimalsBinding, CompetitiveCells, CulturedCytoplasm/metabolismMeiosis/geneticsMiceOocytes/ physiologyOogenesis/geneticsProtein BiosynthesisRNA, Messenger/ physiologyTissue Plasminogen Activator/genetics/metabolism
Identifiers
PMID: 9716406
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STUTZ, A. et al. Masking, unmasking, and regulated polyadenylation cooperate in the translational control of a dormant mRNA in mouse oocytes. In: Genes and Development, 1998, vol. 12, n° 16, p. 2535-2548. https://archive-ouverte.unige.ch/unige:9046

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Deposited on : 2010-07-12

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