en
Scientific article
English

Masking, unmasking, and regulated polyadenylation cooperate in the translational control of a dormant mRNA in mouse oocytes

Published inGenes & development, vol. 12, no. 16, p. 2535-2548
Publication date1998
Abstract

The mechanisms responsible for translational silencing of certain mRNAs in growing oocytes, and for their awakening during meiotic maturation, are not completely elucidated. We show that binding of a approximately 80-kD protein to a UA-rich element in the 3' UTR of tissue-type plasminogen activator mRNA, a mouse oocyte mRNA that is translated during meiotic maturation, silences the mRNA in primary oocytes. Translation can be triggered by injecting a competitor transcript that displaces this silencing factor, without elongation of a pre-existing short poly(A) tail, the presence of which is mandatory. During meiotic maturation, cytoplasmic polyadenylation is necessary to maintain a poly(A) tail, but the determining event for translational activation appears to be the modification or displacement of the silencing factor.

Keywords
  • Animals
  • Binding, Competitive
  • Cells, Cultured
  • Cytoplasm/metabolism
  • Meiosis/genetics
  • Mice
  • Oocytes/ physiology
  • Oogenesis/genetics
  • Protein Biosynthesis
  • RNA, Messenger/ physiology
  • Tissue Plasminogen Activator/genetics/metabolism
Citation (ISO format)
STUTZ, A. et al. Masking, unmasking, and regulated polyadenylation cooperate in the translational control of a dormant mRNA in mouse oocytes. In: Genes & development, 1998, vol. 12, n° 16, p. 2535–2548. doi: 10.1101/gad.12.16.2535
Main files (1)
Article
accessLevelRestricted
Identifiers
ISSN of the journal0890-9369
523views
0downloads

Technical informations

Creation2010/07/12 12:00:51
First validation2010/07/12 12:00:51
Update time2023/03/14 15:53:00
Status update2023/03/14 15:53:00
Last indexation2024/02/12 18:57:35
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack