Masking, unmasking, and regulated polyadenylation cooperate in the translational control of a dormant mRNA in mouse oocytes

Stutz, A.; Conne, Béatrice; Huarte, Joachim; Gubler, P.; Volkel, V.; Flandin, P.; Vassalli, Jean-Dominique

doi:10.1101/gad.12.16.2535

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Title		Masking, unmasking, and regulated polyadenylation cooperate in the translational control of a dormant mRNA in mouse oocytes
Authors		Stutz, A. Conne, Béatrice Huarte, Joachim Gubler, P. Volkel, V. Flandin, P. Vassalli, Jean-Dominique
Published in		Genes and Development. 1998, vol. 12, no. 16, p. 2535-2548
Abstract		The mechanisms responsible for translational silencing of certain mRNAs in growing oocytes, and for their awakening during meiotic maturation, are not completely elucidated. We show that binding of a approximately 80-kD protein to a UA-rich element in the 3' UTR of tissue-type plasminogen activator mRNA, a mouse oocyte mRNA that is translated during meiotic maturation, silences the mRNA in primary oocytes. Translation can be triggered by injecting a competitor transcript that displaces this silencing factor, without elongation of a pre-existing short poly(A) tail, the presence of which is mandatory. During meiotic maturation, cytoplasmic polyadenylation is necessary to maintain a poly(A) tail, but the determining event for translational activation appears to be the modification or displacement of the silencing factor.
Keywords		Animals — Binding, Competitive — Cells, Cultured — Cytoplasm/metabolism — Meiosis/genetics — Mice — Oocytes/ physiology — Oogenesis/genetics — Protein Biosynthesis — RNA, Messenger/ physiology — Tissue Plasminogen Activator/genetics/metabolism
Identifiers		DOI: 10.1101/gad.12.16.2535 PMID: 9716406
Full text		Article - Limited access to UNIGE Other version: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC317088/pdf/x4.pdf
Structures		Faculté de médecine / Section de médecine clinique / Département de médecine
Citation (ISO format)		STUTZ, A. et al. Masking, unmasking, and regulated polyadenylation cooperate in the translational control of a dormant mRNA in mouse oocytes. In: Genes and Development, 1998, vol. 12, n° 16, p. 2535-2548. doi: 10.1101/gad.12.16.2535 https://archive-ouverte.unige.ch/unige:9046