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MAP kinase signaling antagonizes PAR-1 function during polarization of the early Caenorhabditis elegans embryo

Spilker, A. C.
Rabilotta, Alexia
Zbinden, Caroline
Labbe, J. C.
Published in Genetics. 2009, vol. 183, no. 3, p. 965-977
Abstract PAR proteins (partitioning defective) are major regulators of cell polarity and asymmetric cell division. One of the par genes, par-1, encodes a Ser/Thr kinase that is conserved from yeast to mammals. In Caenorhabditis elegans, par-1 governs asymmetric cell division by ensuring the polar distribution of cell fate determinants. However the precise mechanisms by which PAR-1 regulates asymmetric cell division in C. elegans remain to be elucidated. We performed a genomewide RNAi screen and identified six genes that specifically suppress the embryonic lethal phenotype associated with mutations in par-1. One of these suppressors is mpk-1, the C. elegans homolog of the conserved mitogen activated protein (MAP) kinase ERK. Loss of function of mpk-1 restored embryonic viability, asynchronous cell divisions, the asymmetric distribution of cell fate specification markers, and the distribution of PAR-1 protein in par-1 mutant embryos, indicating that this genetic interaction is functionally relevant for embryonic development. Furthermore, disrupting the function of other components of the MAPK signaling pathway resulted in suppression of par-1 embryonic lethality. Our data therefore indicates that MAP kinase signaling antagonizes PAR-1 signaling during early C. elegans embryonic polarization.
Keywords Amino Acid SequenceAnimalsCaenorhabditis elegans/embryology/genetics/ metabolismCaenorhabditis elegans Proteins/genetics/ metabolismCell Polarity/genetics/physiologyEmbryo, Nonmammalian/cytology/embryology/ metabolismEpistasis, GeneticGenome, Helminth/geneticsMAP Kinase Signaling System/genetics/physiologyMicroscopy, ConfocalMitogen-Activated Protein Kinase 1/genetics/ metabolismMolecular Sequence DataMutationProtein-Serine-Threonine Kinases/genetics/ metabolismRNA InterferenceSequence Homology, Amino AcidTime Factors
PMID: 19720857
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SPILKER, A. C. et al. MAP kinase signaling antagonizes PAR-1 function during polarization of the early Caenorhabditis elegans embryo. In: Genetics, 2009, vol. 183, n° 3, p. 965-977. doi: 10.1534/genetics.109.106716 https://archive-ouverte.unige.ch/unige:9036

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Deposited on : 2010-07-12

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