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Scientific article
Open access
English

Allelic mapping bias in RNA-sequencing is not a major confounder in eQTL studies

Published inGenomeBiology.com, vol. 15, no. 9, 467
Publication date2014
Abstract

RNA sequencing (RNA-seq) is the current gold-standard method to quantify gene expression for expression quantitative trait locus (eQTL) studies. However, a potential caveat in these studies is that RNA-seq reads carrying the non-reference allele of variant loci can have lower probability to map correctly to the reference genome, which could bias gene quantifications and cause false positive eQTL associations. In this study, we analyze the effect of this allelic mapping bias in eQTL discovery.

Keywords
  • Chromosome Mapping
  • Genetic Association Studies
  • Human Genome Project
  • Humans
  • Polymorphism, Single Nucleotide
  • Quantitative Trait Loci
  • Sequence Alignment
  • Sequence Analysis, RNA
Citation (ISO format)
PANOUSIS, Nikolaos et al. Allelic mapping bias in RNA-sequencing is not a major confounder in eQTL studies. In: GenomeBiology.com, 2014, vol. 15, n° 9, p. 467. doi: 10.1186/s13059-014-0467-2
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ISSN of the journal1465-6906
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