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Toxoplasma profilin is essential for host cell invasion and TLR11–dependent induction of an interleukin–12 response

Plattner, Fabienne
Yarovinsky, Felix
Romero, Stephane
Didry, Dominique
Carlier, Marie-France
Sher, Alan
Published in Cell Host & Microbe. 2008, vol. 3, no. 2, p. 77-87
Abstract Apicomplexan parasites exhibit actin–dependent gliding motility that is essential for migration across biological barriers and host cell invasion. Profilins are key contributors to actin polymerization, and the parasite Toxoplasma gondii possesses a profilin–like protein that is recognized by Toll–like receptor TLR11 in the host innate immune system. Here, we show by conditional disruption of the corresponding gene that T.gondii profilin, while not required for intracellular growth, is indispensable for gliding motility, host cell invasion, active egress from host cells, and virulence in mice. Furthermore, parasites lacking profilin are unable to induce TLR11–dependent production in vitro and in vivo of the defensive host cytokine interleukin–12. Thus, profilin is an essential element of two aspects of T. gondii infection. Like bacterial flagellin, profilin plays a role in motility while serving as a microbial ligand recognized by the host innate immune system.
Keywords Actins/metabolismAnimalsCell LineCell MovementGenes, ProtozoanGenetic Complementation TestHumansInterleukin–12/biosynthesis/immunologyPlasmodium falciparum/geneticsPoint MutationProfilins/physiologyProtozoan Proteins/physiologyToll–Like Receptors/metabolismToxoplasma/pathogenicity/physiologyToxoplasmosis, Animal/immunology/parasitologyVirulence
PMID: 18312842
Full text
Research group Biologie d'un parasite intracellulaire obligatoire (773)
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PLATTNER, Fabienne et al. Toxoplasma profilin is essential for host cell invasion and TLR11–dependent induction of an interleukin–12 response. In: Cell Host & Microbe, 2008, vol. 3, n° 2, p. 77-87. https://archive-ouverte.unige.ch/unige:902

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Deposited on : 2009-02-24

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