UNIGE document Scientific Article
previous document  unige:90158  next document
add to browser collection
Title

In vivo rapid gene delivery into postmitotic neocortical neurons using iontoporation

Authors
Published in Nature Protocols. 2015, vol. 10, no. 1, p. 25-32
Abstract This protocol describes a method for directing the expression of genes of interest into postmitotic neocortical neurons in vivo. Microinjection of a DNA plasmid-amphiphilic molecule mix into the neocortex followed by delivery of an ad hoc electric pulse protocol during the first few days of life in mice allows rapid, focal and efficient expression of genes in postmitotic neurons. Compared with other gene delivery techniques such as in utero electroporation and viral infection, this method allows rapid (12 h), focal (50-200 μm), mosaic-like (50 to several hundred neurons) targeting of postmitotic neurons within existing circuits. This 'iontoporation' protocol, which can be completed within ∼20 min per mouse, allows straightforward assessment of genetic constructs in postmitotic cortical neurons and subsequent genetic, histological and physiological investigations of gene function.
Keywords AnimalsElectroporation/methodsGene Expression Regulation/physiologyGene Transfer TechniquesIontophoresis/methodsMiceMicroinjectionsNeocortex/cytologyNeurons/metabolism
Identifiers
PMID: 25474030
Full text
Article (Published version) (1.1 MB) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research group Neurobiologie développementale et plasticité (880)
Citation
(ISO format)
DE LA ROSSA, Andrés, JABAUDON, Denis. In vivo rapid gene delivery into postmitotic neocortical neurons using iontoporation. In: Nature Protocols, 2015, vol. 10, n° 1, p. 25-32. https://archive-ouverte.unige.ch/unige:90158

63 hits

0 download

Update

Deposited on : 2016-12-08

Export document
Format :
Citation style :