A compressible scaffold for minimally invasive delivery of large intact neuronal networks

Beduer, Amélie; Braschler, Thomas Michaël; Peric, Oliver; Fantner, Georg E; Mosser, Sébastien; Fraering, Patrick C; Benchérif, Sidi; Mooney, David J; Renaud, Philippe

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Title		A compressible scaffold for minimally invasive delivery of large intact neuronal networks
Authors		Beduer, Amélie Braschler, Thomas Michaël Peric, Oliver Fantner, Georg E Mosser, Sébastien Fraering, Patrick C Benchérif, Sidi Mooney, David J Renaud, Philippe show all authors [1 - 9]
Published in		Advanced Healthcare Materials. 2015, vol. 4, no. 2, p. 301-12
Abstract		Millimeter to centimeter-sized injectable neural scaffolds based on macroporous cryogels are presented. The polymer-scaffolds are made from alginate and carboxymethyl-cellulose by a novel simple one-pot cryosynthesis. They allow surgical sterility by means of autoclaving, and present native laminin as an attachment motive for neural adhesion and neurite development. They are designed to protect an extended, living neuronal network during compression to a small fraction of the original volume in order to enable minimally invasive delivery. The scaffolds behave as a mechanical meta-material: they are soft at the macroscopic scale, enabling injection through narrow-bore tubing and potentially good cellular scaffold integration in soft target tissues such as the brain. At the same time, the scaffold material has a high local Young modulus, allowing protection of the neuronal network during injection. Based on macroscopic and nanomechanical characterization, the generic geometrical and mechanical design rules are presented, enabling macroporous cellular scaffold injectability.
Keywords		Alginates/pharmacology — Carboxymethylcellulose Sodium/pharmacology — Cell Adhesion/drug effects — Cell Line, Tumor — Cell Survival/drug effects — Computer Simulation — Cryogels/pharmacology — Drug Delivery Systems — Finite Element Analysis — Glucuronic Acid/pharmacology — Hexuronic Acids/pharmacology — Humans — Injections — Neurons/cytology — Stress, Mechanical — Tissue Scaffolds/chemistry
Identifiers		DOI: 10.1002/adhm.201400250 PMID: 25178838
Full text		Article (Author postprint) (4.1 MB) - Free access
Structures		Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie
Research groups		Groupe Thomas Braschler (970) LMIS4/EPFL
Projects		FNS: PBELP3-133350 FNS: IZK0Z2_154342
Citation (ISO format)		BEDUER, Amélie et al. A compressible scaffold for minimally invasive delivery of large intact neuronal networks. In: Advanced Healthcare Materials, 2015, vol. 4, n° 2, p. 301-12. https://archive-ouverte.unige.ch/unige:89963