• Home
  1. Home
  2. Cdc48/p97 promotes reformation of the nucleus by extracting the kinase Aurora B from chromatin
Scientific article
English

Cdc48/p97 promotes reformation of the nucleus by extracting the kinase Aurora B from chromatin

Contributors

Ramadan, Kristijan; Bruderer, Roland; Spiga, F. M.; Popp, Oliver; Baur, Tina; Gotta, Monica; Meyer, H. H.

Published in

Nature, vol. 450, no. 7173, p. 1258-1262

Publication date

2007

Abstract

During division of metazoan cells, the nucleus disassembles to allow chromosome segregation, and then reforms in each daughter cell. Reformation of the nucleus involves chromatin decondensation and assembly of the double-membrane nuclear envelope around the chromatin; however, regulation of the process is still poorly understood. In vitro, nucleus formation requires p97 (ref. 3), a hexameric ATPase implicated in membrane fusion and ubiquitin-dependent processes. However, the role and relevance of p97 in nucleus formation have remained controversial. Here we show that p97 stimulates nucleus reformation by inactivating the chromatin-associated kinase Aurora B. During mitosis, Aurora B inhibits nucleus reformation by preventing chromosome decondensation and formation of the nuclear envelope membrane. During exit from mitosis, p97 binds to Aurora B after its ubiquitylation and extracts it from chromatin. This leads to inactivation of Aurora B on chromatin, thus allowing chromatin decondensation and nuclear envelope formation. These data reveal an essential pathway that regulates reformation of the nucleus after mitosis and defines ubiquitin-dependent protein extraction as a common mechanism of Cdc48/p97 activity also during nucleus formation.

Keywords

  • Adenosine Triphosphatases/deficiency/genetics/ metabolism
  • Animals
  • Caenorhabditis elegans
  • Cell Cycle Proteins/genetics/ metabolism
  • Cell Nucleus/enzymology/ metabolism
  • Chromatin/ enzymology
  • Female
  • Male
  • Nuclear Envelope/metabolism
  • Protein-Serine-Threonine Kinases/antagonists & inhibitors/ metabolism
  • RNA Interference
  • Ubiquitin/metabolism
  • Ubiquitination
  • Xenopus laevis

UNIGE affiliation entities

Citation (ISO format)

RAMADAN, Kristijan et al. Cdc48/p97 promotes reformation of the nucleus by extracting the kinase Aurora B from chromatin. In: Nature, 2007, vol. 450, n° 7173, p. 1258–1262. doi: 10.1038/nature06388

Main files (1)

Article

Identifiers

Additional URL for this publication

http://www.nature.com/nature/journal/v450/n7173/pdf/nature06388.pdf

Journal ISSN

0028-0836
668views
0downloads

Technical informations

Creation

12/07/2010 11:59:41

First validation

12/07/2010 11:59:41

Update

14/10/2025 06:05:13

Status update

14/03/2023 15:52:35

Last indexation

03/02/2026 10:28:54
All rights reserved by Archive ouverte UNIGE and the University of Geneva
OpenAIRE Indexed & Validated