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Characterization of YqjM, an Old Yellow Enzyme homolog from Bacillus subtilis involved in the oxidative stress response

Amrhein, Nikolaus
Macheroux, Peter
Published in Journal of Biological Chemistry. 2003, vol. 278, no. 22, p. 19891-7
Abstract In this paper, we demonstrate that a protein from Bacillus subtilis (YqjM) shares many characteristic biochemical properties with the homologous yeast Old Yellow Enzyme (OYE); the enzyme binds FMN tightly but noncovalently, preferentially uses NADPH as a source of reducing equivalents, and forms charge transfer complexes with phenolic compounds such as p-hydroxybenzaldehyde. Like yeast OYE and other members of the family, YqjM catalyzes the reduction of the double bond of an array of alpha,beta-unsaturated aldehydes and ketones including nitroester and nitroaromatic compounds. Although yeast OYE was the first member of this family to be discovered in 1933 and was the first flavoenzyme ever to be isolated, the physiological role of the family still remains obscure. The finding that alpha,beta-unsaturated compounds are substrates provoked speculation that the OYE family might be involved in reductive degradation of xenobiotics or lipid peroxidation products. Here, for the first time, we demonstrate on the protein level that whereas YqjM shows a basal level of expression in B. subtilis, the addition of the toxic xenobiotic, trinitrotoluene, leads to a rapid induction of the protein in vivo denoting a role in detoxification. Moreover, we show that YqjM is rapidly induced in response to oxidative stress as exerted by hydrogen peroxide, demonstrating a potential physiological role for this enigmatic class of proteins.
Keywords Bacillus subtilis/enzymologyCatalysisCloningMolecularNADPH Dehydrogenase/genetics/isolation & purification/metabolismOxidative StressSpectrum Analysis
PMID: 12660247
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FITZPATRICK, Thérésa Bridget, AMRHEIN, Nikolaus, MACHEROUX, Peter. Characterization of YqjM, an Old Yellow Enzyme homolog from Bacillus subtilis involved in the oxidative stress response. In: Journal of Biological Chemistry, 2003, vol. 278, n° 22, p. 19891-7. doi: 10.1074/jbc.M211778200

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Deposited on : 2016-11-29

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