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Title

Structure and characterization of the glycan moiety of L-amino-acid oxidase from the Malayan pit viper Calloselasma rhodostoma

Authors
Geyer, A
Pawelek, P D
Kitzing, K
Vrielink, A
Ghisla, S
Macheroux, P
Published in European Journal of Biochemistry. 2001, vol. 268, no. 14, p. 4044-4053
Abstract Ophidian L-amino-acid oxidase (L-amino-acid oxygen:oxidoreductase, deaminating, EC 1.4.3.2) is found in the venom of many poisonous snakes (crotalids, elapids and viperids). This FAD-dependent glycoprotein has been studied from several snake species (e.g. Crotalus adamanteus, Crotalus atrox and Calloselasma rhodostoma) in detail with regard to the biochemical and enzymatic properties. The nature of glycosylation, however, as well as the chemical structure(s) of the attached oligosaccharide(s) are unknown. In view of the putative involvement of the glycan moiety in the biological effects of ophidian L-amino-acid oxidase, notably the apoptotic activity of the enzyme, structural knowledge is needed to evaluate its exact function. In this study we report on the glycosylation of L-amino-acid oxidase from the venom of the Malayan pit viper (Calloselasma rhodostoma). Its glycosylation is remarkably homogeneous with the major oligosaccharide accounting for approximately 90% of the total sugar content. Based on detailed analysis of the isolated oligosaccharide by 2D NMR spectroscopies and MALDI-TOF mass spectrometry the glycan is identified as a bis-sialylated, biantennary, core-fucosylated dodecasaccharide. The biological significance of this finding is discussed in light of the biological activities of the enzyme.
Keywords Amino Acid Oxidoreductases/chemistryAnimalsCarbohydrate SequenceCrotalid Venoms/enzymologyGlycoproteins/chemistryL-Amino Acid OxidaseMass SpectrometryModelsMolecularMolecular Sequence DataNuclear Magnetic ResonanceBiomolecularOligosaccharides/chemistryViperidae
Identifiers
PMID: 11453999
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GEYER, A et al. Structure and characterization of the glycan moiety of L-amino-acid oxidase from the Malayan pit viper Calloselasma rhodostoma. In: European Journal of Biochemistry, 2001, vol. 268, n° 14, p. 4044-4053. https://archive-ouverte.unige.ch/unige:89524

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Deposited on : 2016-11-29

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