Scientific article
Open access

Glioma Stemlike Cells Enhance the Killing of Glioma Differentiated Cells by Cytotoxic Lymphocytes

Published inPloS one, vol. 11, no. 4, e0153433
Publication date2016

Glioblastoma multiforme, the most aggressive primary brain tumor, is maintained by a subpopulation of glioma cells with self-renewal properties that are able to recapitulate the entire tumor even after surgical resection or chemo-radiotherapy. This typifies the vast heterogeneity of this tumor with the two extremes represented on one end by the glioma stemlike cells (GSC) and on the other by the glioma differentiated cells (GDC). Interestingly, GSC are more sensitive to immune effector cells than the GDC counterpart. However, how GSC impact on the killing on the GDC and vice versa is not clear. Using a newly developed cytotoxicity assay allowing to simultaneously monitor cytotoxic lymphocytes-mediated killing of GSC and GDC, we found that although GSC were always better killed and that their presence enhanced the killing of GDC. In contrast, an excess of GDC had a mild protective effect on the killing of GSC, depending on the CTL type. Overall, our results suggest that during combination therapy, immunotherapy would be the most effective after prior treatment with conventional therapies.

  • Animals
  • Cell Death/physiology
  • Cell Differentiation/physiology
  • Cell Line, Tumor
  • Glioblastoma/pathology
  • Glioma/pathology
  • Humans
  • Lymphocytes/pathology
  • Mice
  • Neoplastic Stem Cells/pathology
Citation (ISO format)
BASSOY, Esen et al. Glioma Stemlike Cells Enhance the Killing of Glioma Differentiated Cells by Cytotoxic Lymphocytes. In: PloS one, 2016, vol. 11, n° 4, p. e0153433. doi: 10.1371/journal.pone.0153433
Main files (1)
Article (Published version)
ISSN of the journal1932-6203

Technical informations

Creation11/23/2016 2:36:00 PM
First validation11/23/2016 2:36:00 PM
Update time03/15/2023 1:00:42 AM
Status update03/15/2023 1:00:41 AM
Last indexation02/12/2024 11:26:19 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack