Scientific article
English

Detection of mRNA for proteins involved in retinol metabolism in iris pigment epithelium

Published inGraefe's archive for clinical and experimental ophthalmology, vol. 237, no. 12, p. 1046-1051
Publication date1999
Abstract

To investigate in iris pigment epithelium (IPE) the expression of mRNA for proteins involved in retinol metabolism we used a semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) technique.

Keywords
  • Aldehyde Oxidoreductases/biosynthesis/genetics
  • Animals
  • Antibodies, Monoclonal
  • Carrier Proteins/biosynthesis/genetics
  • Cattle
  • DNA Primers/chemistry
  • Eye Proteins/biosynthesis/genetics
  • Fluorescent Antibody Technique, Indirect
  • Gene Expression
  • Glyceraldehyde-3-Phosphate Dehydrogenases/biosynthesis/genetics
  • Iris/cytology/metabolism
  • Membrane Proteins
  • Phosphoproteins/biosynthesis/genetics
  • Pigment Epithelium of Eye/cytology/metabolism
  • RNA, Messenger/biosynthesis
  • Retinal Dehydrogenase
  • Retinaldehyde/biosynthesis/genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Trans-Activators
  • Vitamin A/metabolism
  • Beta 2-Microglobulin/biosynthesis/genetics
Affiliation entities Not a UNIGE publication
Citation (ISO format)
THUMANN, Gabriele et al. Detection of mRNA for proteins involved in retinol metabolism in iris pigment epithelium. In: Graefe’s archive for clinical and experimental ophthalmology, 1999, vol. 237, n° 12, p. 1046–1051. doi: 10.1007/s004170050343
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
Journal ISSN0721-832X
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