Scientific article
English

Presence of xenogenic mouse RNA in RPE and IPE cells cultured on mitotically inhibited 3T3 fibroblasts

Published in

Investigative ophthalmology & visual science, vol. 52, no. 5, p. 2817-2824

Publication date

2011

Abstract

Mitotically inhibited 3T3 fibroblasts are used as feeder layers to culture a variety of cells. However, transplantation of human cells cultured on mitotically arrested mouse cells poses potential risks, such as disease transfer and contamination with 3T3 cells. Bovine RPE and IPE cells were cultured on mitomycin-treated 3T3 fibroblasts, to examine cell characteristics and contamination by 3T3 products.

Keywords

  • Alkylating Agents/pharmacology
  • Animals
  • Cattle
  • Cell Proliferation/drug effects
  • Cell Survival
  • Coculture Techniques
  • Collagen/genetics
  • Collagen Type I
  • Fibroblasts/cytology/metabolism
  • Fluorescent Antibody Technique, Indirect
  • Gene Expression Profiling
  • Iris/cytology
  • Membrane Proteins/genetics/metabolism
  • Mice
  • Mitomycin/pharmacology
  • Mitosis/drug effects
  • NIH 3T3 Cells
  • Phagocytosis/physiology
  • Phosphoproteins/genetics/metabolism
  • Pigment Epithelium of Eye/cytology/physiology
  • RNA, Messenger/metabolism
  • Retinal Pigment Epithelium/cytology/physiology
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rod Cell Outer Segment/metabolism
  • Swine
  • Tight Junctions
  • Zonula Occludens-1 Protein

UNIGE affiliation entities

Not a UNIGE publication

Citation (ISO format)

JOHNEN, Sandra et al. Presence of xenogenic mouse RNA in RPE and IPE cells cultured on mitotically inhibited 3T3 fibroblasts. In: Investigative ophthalmology & visual science, 2011, vol. 52, n° 5, p. 2817–2824. doi: 10.1167/iovs.10-6429

Main files (1)

Article (Published version)

Identifiers

Journal ISSN

0146-0404
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Technical informations

Creation

21/09/2016 13:04:00

First validation

21/09/2016 13:04:00

Update

15/03/2023 00:59:19

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