en
Scientific article
English

Presence of xenogenic mouse RNA in RPE and IPE cells cultured on mitotically inhibited 3T3 fibroblasts

Published inInvestigative ophthalmology & visual science, vol. 52, no. 5, p. 2817-2824
Publication date2011
Abstract

Mitotically inhibited 3T3 fibroblasts are used as feeder layers to culture a variety of cells. However, transplantation of human cells cultured on mitotically arrested mouse cells poses potential risks, such as disease transfer and contamination with 3T3 cells. Bovine RPE and IPE cells were cultured on mitomycin-treated 3T3 fibroblasts, to examine cell characteristics and contamination by 3T3 products.

Keywords
  • Alkylating Agents/pharmacology
  • Animals
  • Cattle
  • Cell Proliferation/drug effects
  • Cell Survival
  • Coculture Techniques
  • Collagen/genetics
  • Collagen Type I
  • Fibroblasts/cytology/metabolism
  • Fluorescent Antibody Technique, Indirect
  • Gene Expression Profiling
  • Iris/cytology
  • Membrane Proteins/genetics/metabolism
  • Mice
  • Mitomycin/pharmacology
  • Mitosis/drug effects
  • NIH 3T3 Cells
  • Phagocytosis/physiology
  • Phosphoproteins/genetics/metabolism
  • Pigment Epithelium of Eye/cytology/physiology
  • RNA, Messenger/metabolism
  • Retinal Pigment Epithelium/cytology/physiology
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rod Cell Outer Segment/metabolism
  • Swine
  • Tight Junctions
  • Zonula Occludens-1 Protein
Affiliation Not a UNIGE publication
Citation (ISO format)
JOHNEN, Sandra et al. Presence of xenogenic mouse RNA in RPE and IPE cells cultured on mitotically inhibited 3T3 fibroblasts. In: Investigative ophthalmology & visual science, 2011, vol. 52, n° 5, p. 2817–2824. doi: 10.1167/iovs.10-6429
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal0146-0404
463views
0downloads

Technical informations

Creation09/21/2016 1:04:00 PM
First validation09/21/2016 1:04:00 PM
Update time03/15/2023 12:59:19 AM
Status update03/15/2023 12:59:18 AM
Last indexation01/16/2024 10:23:29 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack