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Scientific article
English

Endogenic regulation of proliferation and zinc transporters by pigment epithelial cells nonvirally transfected with PEDF

Published inInvestigative ophthalmology & visual science, vol. 52, no. 8, p. 5400-5407
Publication date2011
Abstract

Genetic modification of cells before transplantation may allow the delivery of neuroprotective and other functional molecules to patients with neurodegenerative diseases. To avoid complications associated with virally transfected cells, we have explored the use of nonviral methods to insert genetic material into RPE cells.

Keywords
  • Animals
  • Blotting, Western
  • Carrier Proteins
  • Cation Transport Proteins/metabolism
  • Cell Proliferation
  • Cells, Cultured
  • Electroporation
  • Enzyme-Linked Immunosorbent Assay
  • Eye Proteins/genetics
  • Flow Cytometry
  • Gene Expression Regulation/physiology
  • Green Fluorescent Proteins/metabolism
  • Humans
  • Nerve Growth Factors/genetics
  • Plasmids
  • Rabbits
  • Recombinant Fusion Proteins/metabolism
  • Retinal Pigment Epithelium/cytology/metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Serpins/genetics
  • Transfection
  • Zinc/metabolism
Affiliation Not a UNIGE publication
Citation (ISO format)
JOHNEN, Sandra et al. Endogenic regulation of proliferation and zinc transporters by pigment epithelial cells nonvirally transfected with PEDF. In: Investigative ophthalmology & visual science, 2011, vol. 52, n° 8, p. 5400–5407. doi: 10.1167/iovs.10-6178
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal0146-0404
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