Endogenic regulation of proliferation and zinc transporters by pigment epithelial cells nonvirally transfected with PEDF

Johnen, Sandra; Kazanskaya, Olga; Armogan, Narendra; Stickelmann, Christiane; Stöcker, Michael; Walter, Peter; Thumann, Gabriele

doi:10.1167/iovs.10-6178

Scientific article

English

Endogenic regulation of proliferation and zinc transporters by pigment epithelial cells nonvirally transfected with PEDF

ContributorsJohnen, Sandra; Kazanskaya, Olga; Armogan, Narendra; Stickelmann, Christiane; Stöcker, Michael; Walter, Peter; Thumann, Gabriele

Published inInvestigative ophthalmology & visual science, vol. 52, no. 8, p. 5400-5407

Publication date2011

Abstract

Genetic modification of cells before transplantation may allow the delivery of neuroprotective and other functional molecules to patients with neurodegenerative diseases. To avoid complications associated with virally transfected cells, we have explored the use of nonviral methods to insert genetic material into RPE cells.

Keywords

Animals
Blotting, Western
Carrier Proteins
Cation Transport Proteins/metabolism
Cell Proliferation
Cells, Cultured
Electroporation
Enzyme-Linked Immunosorbent Assay
Eye Proteins/genetics
Flow Cytometry
Gene Expression Regulation/physiology
Green Fluorescent Proteins/metabolism
Humans
Nerve Growth Factors/genetics
Plasmids
Rabbits
Recombinant Fusion Proteins/metabolism
Retinal Pigment Epithelium/cytology/metabolism
Reverse Transcriptase Polymerase Chain Reaction
Serpins/genetics
Transfection
Zinc/metabolism

Affiliation entities

Not a UNIGE publication

Citation (ISO format)

JOHNEN, Sandra et al. Endogenic regulation of proliferation and zinc transporters by pigment epithelial cells nonvirally transfected with PEDF. In: Investigative ophthalmology & visual science, 2011, vol. 52, n° 8, p. 5400–5407. doi: 10.1167/iovs.10-6178

Article (Published version)

Identifiers

PID : unige:89305
DOI : 10.1167/iovs.10-6178
PMID : 21508100

Journal ISSN0146-0404

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Endogenic regulation of proliferation and zinc transporters by pigment epithelial cells nonvirally transfected with PEDF

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