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Title

Endogenic regulation of proliferation and zinc transporters by pigment epithelial cells nonvirally transfected with PEDF.

Authors
Johnen, Sandra
Kazanskaya, Olga
Armogan, Narendra
Stickelmann, Christiane
Stöcker, Michael
Walter, Peter
Published in Investigative Ophthalmology & Visual Science. 2011, vol. 52, no. 8, p. 5400-5407
Abstract Genetic modification of cells before transplantation may allow the delivery of neuroprotective and other functional molecules to patients with neurodegenerative diseases. To avoid complications associated with virally transfected cells, we have explored the use of nonviral methods to insert genetic material into RPE cells.
Keywords AnimalsBlotting, WesternCarrier ProteinsCation Transport Proteins/metabolismCell ProliferationCells, CulturedElectroporationEnzyme-Linked Immunosorbent AssayEye Proteins/geneticsFlow CytometryGene Expression Regulation/physiologyGreen Fluorescent Proteins/metabolismHumansNerve Growth Factors/geneticsPlasmidsRabbitsRecombinant Fusion Proteins/metabolismRetinal Pigment Epithelium/cytology/metabolismReverse Transcriptase Polymerase Chain ReactionSerpins/geneticsTransfectionZinc/metabolism
Identifiers
PMID: 21508100
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JOHNEN, Sandra et al. Endogenic regulation of proliferation and zinc transporters by pigment epithelial cells nonvirally transfected with PEDF. In: Investigative Ophthalmology & Visual Science, 2011, vol. 52, n° 8, p. 5400-5407. https://archive-ouverte.unige.ch/unige:89305

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Deposited on : 2016-11-23

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