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Protease nexin 1 in the murine kidney: glomerular localization and up-regulation in glomerulopathies

Schaeren-Wiemers, N.
Pastore, Y.
Fulpius, T.
Monard, D.
Schifferli, J. A.
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Published in Kidney International. 1996, vol. 50, no. 6, p. 1936-1945
Abstract Protease nexin 1 (PN-1), a potent serpin-class antiprotease, is thought to be synthesized in the murine kidney. However, neither the cellular localization of PN-1 synthesis nor its role has yet been defined. To address these questions, we determined by in situ hybridizations RNase protection assay and immunoblotting, the sites of PN-1 mRNA accumulation in normal mouse kidneys and the modulation of PN-1 expression in several pathological conditions. In normal kidneys, PN-1 mRNA was detected primarily in glomeruli, most likely in mesangial cells. The glomerular expression of PN-1 was substantially enhanced not only in lupus-like glomerulonephritis (induced by IgG3 monoclonal rheumatoid factors or occurring spontaneously in lupus-prone mice), but also in mild glomerular lesions associated with intracapillary thrombi induced by IgG3 anti-trinitrophenyl monoclonal antibodies. In contrast, no modulation of PN-1 mRNA levels was observed during the course of lipopolysaccharide-induced acute tubular necrosis. A constitutive PN-1 gene expression and its up-regulation during glomerular injury suggest a possible role for PN-1 in glomerular biology. In view of its high inhibitory activity towards thrombin, mesangial PN-1 may be involved in the control of glomerular coagulation following initial glomerular injuries.
Keywords Amyloid beta-Protein PrecursorAnimalsCarrier Proteins/analysis/ geneticsGene Expression RegulationGlomerulonephritis/ metabolismKidney Glomerulus/ metabolismLupus Nephritis/metabolismMiceMice, Inbred StrainsPlasminogen Activator Inhibitor 1/geneticsRNA, Messenger/analysisReceptors, Cell SurfaceSerine Proteinase Inhibitors/ geneticsUp-Regulation
PMID: 8943477
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MOLL, Solange et al. Protease nexin 1 in the murine kidney: glomerular localization and up-regulation in glomerulopathies. In: Kidney International, 1996, vol. 50, n° 6, p. 1936-1945. https://archive-ouverte.unige.ch/unige:8912

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Deposited on : 2010-07-12

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