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Scientific article
English

Protease nexin 1 in the murine kidney: glomerular localization and up-regulation in glomerulopathies

Published inKidney international, vol. 50, no. 6, p. 1936-1945
Publication date1996
Abstract

Protease nexin 1 (PN-1), a potent serpin-class antiprotease, is thought to be synthesized in the murine kidney. However, neither the cellular localization of PN-1 synthesis nor its role has yet been defined. To address these questions, we determined by in situ hybridizations RNase protection assay and immunoblotting, the sites of PN-1 mRNA accumulation in normal mouse kidneys and the modulation of PN-1 expression in several pathological conditions. In normal kidneys, PN-1 mRNA was detected primarily in glomeruli, most likely in mesangial cells. The glomerular expression of PN-1 was substantially enhanced not only in lupus-like glomerulonephritis (induced by IgG3 monoclonal rheumatoid factors or occurring spontaneously in lupus-prone mice), but also in mild glomerular lesions associated with intracapillary thrombi induced by IgG3 anti-trinitrophenyl monoclonal antibodies. In contrast, no modulation of PN-1 mRNA levels was observed during the course of lipopolysaccharide-induced acute tubular necrosis. A constitutive PN-1 gene expression and its up-regulation during glomerular injury suggest a possible role for PN-1 in glomerular biology. In view of its high inhibitory activity towards thrombin, mesangial PN-1 may be involved in the control of glomerular coagulation following initial glomerular injuries.

Keywords
  • Amyloid beta-Protein Precursor
  • Animals
  • Carrier Proteins/analysis/ genetics
  • Gene Expression Regulation
  • Glomerulonephritis/ metabolism
  • Kidney Glomerulus/ metabolism
  • Lupus Nephritis/metabolism
  • Mice
  • Mice, Inbred Strains
  • Plasminogen Activator Inhibitor 1/genetics
  • RNA, Messenger/analysis
  • Receptors, Cell Surface
  • Serine Proteinase Inhibitors/ genetics
  • Up-Regulation
Citation (ISO format)
MOLL, Solange et al. Protease nexin 1 in the murine kidney: glomerular localization and up-regulation in glomerulopathies. In: Kidney international, 1996, vol. 50, n° 6, p. 1936–1945.
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ISSN of the journal0085-2538
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