Scientific article
Open access

In-phase oscillation of global regulons is orchestrated by a pole-specific organizer

Publication date2016

Cell fate determination in the asymmetric bacterium Caulobacter crescentus (Caulobacter) is triggered by the localization of the developmental regulator SpmX to the old (stalked) cell pole during the G1→S transition. Although SpmX is required to localize and activate the cell fate-determining kinase DivJ at the stalked pole in Caulobacter, in cousins such as Asticcacaulis, SpmX directs organelle (stalk) positioning and possibly other functions. We define the conserved σ(54)-dependent transcriptional activator TacA as a global regulator in Caulobacter whose activation by phosphorylation is indirectly down-regulated by SpmX. Using a combination of forward genetics and cytological screening, we uncover a previously uncharacterized and polarized component (SpmY) of the TacA phosphorylation control system, and we show that SpmY function and localization are conserved. Thus, SpmX organizes a site-specific, ancestral, and multifunctional regulatory hub integrating the in-phase oscillation of two global transcriptional regulators, CtrA (the master cell cycle transcriptional regulator A) and TacA, that perform important cell cycle functions.

Citation (ISO format)
JANAKIRAMAN, Balaganesh et al. In-phase oscillation of global regulons is orchestrated by a pole-specific organizer. In: Proceedings of the National Academy of Sciences of the United States of America, 2016, vol. 113, n° 44, p. 12550–12555. doi: 10.1073/pnas.1610723113
Main files (1)
Article (Published version)
ISSN of the journal0027-8424

Technical informations

Creation10/30/2016 8:39:00 AM
First validation10/30/2016 8:39:00 AM
Update time03/15/2023 12:57:11 AM
Status update03/15/2023 12:57:11 AM
Last indexation05/02/2024 5:56:12 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack