UNIGE document Scientific Article
previous document  unige:89083  next document
add to browser collection
Title

In-phase oscillation of global regulons is orchestrated by a pole-specific organizer

Authors
Janakiraman, Balaganesh
Narayanan, Sharath
Published in Proceedings of the National Academy of Sciences. 2016, vol. 113, no. 44, p. 12550-12555
Abstract Cell fate determination in the asymmetric bacterium Caulobacter crescentus (Caulobacter) is triggered by the localization of the developmental regulator SpmX to the old (stalked) cell pole during the G1→S transition. Although SpmX is required to localize and activate the cell fate-determining kinase DivJ at the stalked pole in Caulobacter, in cousins such as Asticcacaulis, SpmX directs organelle (stalk) positioning and possibly other functions. We define the conserved σ(54)-dependent transcriptional activator TacA as a global regulator in Caulobacter whose activation by phosphorylation is indirectly down-regulated by SpmX. Using a combination of forward genetics and cytological screening, we uncover a previously uncharacterized and polarized component (SpmY) of the TacA phosphorylation control system, and we show that SpmY function and localization are conserved. Thus, SpmX organizes a site-specific, ancestral, and multifunctional regulatory hub integrating the in-phase oscillation of two global transcriptional regulators, CtrA (the master cell cycle transcriptional regulator A) and TacA, that perform important cell cycle functions.
Identifiers
PMID: 27791133
Full text
Article (Published version) (1.3 MB) - public document Free access
Structures
Research group Régulation du cycle cellulaire dans C.crescentus, une bactérie asymétrique (895)
Citation
(ISO format)
JANAKIRAMAN, Balaganesh et al. In-phase oscillation of global regulons is orchestrated by a pole-specific organizer. In: Proceedings of the National Academy of Sciences, 2016, vol. 113, n° 44, p. 12550-12555. https://archive-ouverte.unige.ch/unige:89083

77 hits

26 downloads

Update

Deposited on : 2016-11-18

Export document
Format :
Citation style :