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Basic Techniques to Assess Seed Germination Responses to Abiotic Stress in Arabidopsis thaliana

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Published in Caveolae : methods and protocols: Springer. 2016
Collection Methods in Molecular Biology; 1398
Abstract The model organism Arabidopsis thaliana has been extensively used to unmask the molecular genetic signaling pathways controlling seed germination in plants. In Arabidopsis, the normal seed to seedling developmental transition involves testa rupture soon followed by endosperm rupture, radicle elongation, root hair formation, cotyledon expansion, and greening. Here we detail a number of basic procedures to assess Arabidopsis seed germination in response to different light (red and far-red pulses), temperature (seed thermoinhibition), and water potential (osmotic stress) environmental conditions. We also discuss the role of the endosperm and how its germination-repressive activity can be monitored genetically by means of a seed coat bedding assay. Finally we detail how to evaluate germination responses to changes in gibberellin (GA) and abscisic acid (ABA) levels by manipulating pharmacologically the germination medium.
Keywords Abscisic Acid/pharmacologyArabidopsis/drug effects/metabolismArabidopsis Proteins/metabolismEndosperm/drug effects/metabolismGene Expression Regulation, Plant/drug effectsGermination/drug effectsGibberellins/pharmacologySeeds/drug effects/metabolism
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PMID: 26867624
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Research group Groupe Lopez-Molina
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PISKUREWICZ, Urszula, LOPEZ MOLINA, Luis. Basic Techniques to Assess Seed Germination Responses to Abiotic Stress in Arabidopsis thaliana. In: Environmental Responses in Plants : Methods and protocols. [s.l.] : Springer, 2016. (Methods in Molecular Biology; 1398) doi: 10.1007/978-1-4939-3356-3_15 https://archive-ouverte.unige.ch/unige:88988

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Deposited on : 2016-11-16

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