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Compartmentalization of the endoplasmic reticulum in the early C. elegans embryos

Published inThe Journal of cell biology, vol. 214, no. 6, p. 665-676
Publication date2016
Abstract

The one-cell Caenorhabditis elegans embryo is polarized to partition fate determinants between the cell lineages generated during its first division. Using fluorescence loss in photobleaching, we find that the endoplasmic reticulum (ER) of the C. elegans embryo is physically continuous throughout the cell, but its membrane is compartmentalized shortly before nuclear envelope breakdown into an anterior and a posterior domain, indicating that a diffusion barrier forms in the ER membrane between these two domains. Using mutants with disorganized ER, we show that ER compartmentalization is independent of the morphological transition that the ER undergoes in mitosis. In contrast, compartmentalization takes place at the position of the future cleavage plane in a par-3-dependent manner. Together, our data indicate that the ER membrane is compartmentalized in cells as diverse as budding yeast, mouse neural stem cells, and the early C. elegans embryo.

Citation (ISO format)
LEE, Zuo Yen et al. Compartmentalization of the endoplasmic reticulum in the early C. elegans embryos. In: The Journal of cell biology, 2016, vol. 214, n° 6, p. 665–676. doi: 10.1083/jcb.201601047
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ISSN of the journal0021-9525
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