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Response to Pick

Rutter, A Richard
Published in Antioxidants & Redox Signaling. 2015, vol. 23, no. 15, p. 1251-1253
Abstract In his letter, Dr. Pick criticizes our use of relative values when representing the NOX2 inhibitory action of a novel small molecule (GSK2795039) in a semi-recombinant NOX2 membrane assay. To address this concern, we performed additional experiments using the superoxide inhibitable assays cytochrome C and water soluble tetrazolium salt (WST-1) reduction. In this letter, we document turnover values between 80 and 100 mol O2(•-)/s/mol cytochrome b558 in our semi-recombinant assay and confirmed that GSK2795039 inhibits the NOX2 isoform in the submicromolar range. Antioxid. Redox Signal. 23, 1251-1253.
Keywords Aminopyridines/pharmacologyAnimalsDrug DiscoveryEnzyme Inhibitors/pharmacologyMaleMembrane Glycoproteins/metabolismNADPH Oxidase/metabolismSulfonamides/pharmacology
PMID: 26566578
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Research group Radicaux libres et cellules souches embryonnaires (60)
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JAQUET, Vincent, RUTTER, A Richard. Response to Pick. In: Antioxidants & redox signaling, 2015, vol. 23, n° 15, p. 1251-1253. doi: 10.1089/ars.2015.6565 https://archive-ouverte.unige.ch/unige:87948

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Deposited on : 2016-10-04

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