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Title

The murine orthologue of the Golgi-localized TPTE protein provides clues to the evolutionary history of the human TPTE gene family

Authors
Scamuffa, N.
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Published in Human Genetics. 2001, vol. 109, no. 6, p. 569-575
Collection Open Access - Licence nationale Springer
Abstract The human TPTE gene encodes a testis-specific protein that contains four potential transmembrane domains and a protein tyrosine phosphatase motif, and shows homology to the tumor suppressor PTEN/MMAC1. Chromosomal mapping revealed multiple copies of the TPTE gene present on the acrocentric chromosomes 13, 15, 21 and 22, and the Y chromosome. Zooblot analysis suggests that mice may possess only one copy of TPTE. In the present study, we report the isolation and initial characterization of the full-length cDNA of the mouse homologue Tpte. At least three different mRNA transcripts ( Tpte.a, b, c) are produced via alternative splicing, encoding predicted proteins that would contain four potential transmembrane domains and a protein tyrosine phosphatase motif. Transfection of a 5'EGFP-TPTE fusion protein in Hela cells revealed an intracellular localization within the Golgi apparatus. Tpte was mapped by radiation hybrid to a region of mouse chromosome 8 that shows conserved synteny with human 13q14.2-q21 between NEK3 and SGT1. This region of the human genome was found to contain a partial, highly diverged copy of TPTE that is likely to represent the ancestral copy from which the other copies of TPTE arose through duplication events. The Y chromosome copy of TPTE is a pseudogene and is not therefore involved in the testis expression of this gene family.
Keywords Amino Acid MotifsAmino Acid SequenceAnimalsCell CompartmentationChromosome MappingChromosomes, Human, Pair 13Evolution, MolecularGolgi Apparatus/ chemistryHumansMaleMembrane Proteins/ geneticsMiceMolecular Sequence DataMultigene Family/ geneticsPTEN PhosphohydrolasePhosphoric Monoester Hydrolases/geneticsPhylogenyProtein Tyrosine PhosphatasesSequence Homology, Amino AcidTumor Suppressor Proteins/geneticsVertebrates/classification/genetics
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PMID: 11810268
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GUIPPONI, Michel et al. The murine orthologue of the Golgi-localized TPTE protein provides clues to the evolutionary history of the human TPTE gene family. In: Human Genetics, 2001, vol. 109, n° 6, p. 569-575. https://archive-ouverte.unige.ch/unige:8775

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Deposited on : 2010-07-12

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