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Identification of a novel member of the CLIC family, CLIC6, mapping to 21q22.12

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Published in Gene. 2003, vol. 320, p. 31-40
Abstract Five members of the newly identified chloride intracellular channel (CLIC) gene family of intracellular chloride channels (CLIC1-CLIC5) have previously been described in humans. Here we report the molecular cloning and initial characterisation of two splice forms of a novel member of this family, CLIC6, mapping to human chromosome 21. Two essential features distinguish CLIC6 from other members of the family. The CLIC6 protein is significantly longer and the CLIC6 gene contains a GC rich segment, which encodes a 10 amino acid motif repeated 14 times in the amino-terminus. Surprisingly, the repeat is conserved in the lagomorphs, but not in the rodents lineage. The putative bovine orthologue of CLIC5, p64, also exhibits a repeated motif, which is different from that of CLIC6. Attempts to functionally characterise CLIC6 by voltage clamp failed to show any chloride channel activity. Hence, the exact function of this protein remains unknown.
Keywords Alternative SplicingAmino Acid SequenceAnimalsCOS CellsCell LineCercopithecus aethiopsChloride Channels/ geneticsChromosome MappingChromosomes, Human, Pair 21/ geneticsCloning, MolecularDNA, Complementary/chemistry/geneticsFemaleGene ExpressionGreen Fluorescent ProteinsHumansLuminescent Proteins/genetics/metabolismMembrane Potentials/physiologyMiceMice, Inbred BALB CMice, Inbred C57BLMice, Inbred CBAMice, Inbred StrainsMicroscopy, FluorescenceMolecular Sequence DataOocytes/metabolism/physiologyRecombinant Fusion Proteins/genetics/metabolismRepetitive Sequences, Nucleic Acid/geneticsSequence AlignmentSequence Analysis, DNASequence Homology, Amino AcidTransfectionXenopus
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PMID: 14597386
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FRIEDLI, Marc et al. Identification of a novel member of the CLIC family, CLIC6, mapping to 21q22.12. In: Gene, 2003, vol. 320, p. 31-40. https://archive-ouverte.unige.ch/unige:8747

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Deposited on : 2010-07-12

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