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Title

Not5-dependent co-translational assembly of Ada2 and Spt20 is essential for functional integrity of SAGA
Author
Kassem, Sari
Director
Collart, Martine
Defense Thèse de doctorat : Univ. Genève, 2016 - Sc. 4941 - 2016/06/22
Abstract Acetylation of histones regulates gene expression in eukaryotes. In the yeast S.cerevisiae it depends mainly upon the ADA and SAGA histone acetyltransferase (HAT) complexes for which Gcn5 is the catalytic subunit. Previous screens have determined that global acetylation is reduced in cells lacking subunits of the Ccr4-Not complex, a global regulator of eukaryotic gene expression. In this study we have characterized the functional connection between the Ccr4-Not complex and SAGA. We show that SAGA mRNAs encoding a core set of SAGA subunits are tethered together for co-translational assembly of the encoded proteins. The Ccr4-Not complex is present at this site to promote the co-translational assembly of these subunits and this is needed for integrity of SAGA. In addition, we determine that a glycolytic enzyme, the glyceraldehyde-3-phosphate dehydrogenase Tdh3, a prototypical moonlighting protein, is tethered at this site of Ccr4-Not-dependent co-translational SAGA assembly and functions as a chaperone.
Keywords Ccr4-Not complex — Not5 — Ada2 — Spt20 — Gcn5 — SAGA — Tdh3 — Co-translational complex assembly
Identifiers
URN: urn:nbn:ch:unige-865979
Full text
Thesis (5.3 MB) - public document Free access
Structures
Faculté des sciences / Section de biologie / Département de biologie cellulaire
Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire
Research group Caractérisation génétique et biochimique du complexe Ccr4-Not conservé (81)
Citation
(ISO format) 		KASSEM, Sari. Not5-dependent co-translational assembly of Ada2 and Spt20 is essential for functional integrity of SAGA. Université de Genève. Thèse, 2016. doi: 10.13097/archive-ouverte/unige:86597 https://archive-ouverte.unige.ch/unige:86597

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Deposited on : 2016-09-05

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