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Not5-dependent co-translational assembly of Ada2 and Spt20 is essential for functional integrity of SAGA

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Defense Thèse de doctorat : Univ. Genève, 2016 - Sc. 4941 - 2016/06/22
Abstract Acetylation of histones regulates gene expression in eukaryotes. In the yeast S.cerevisiae it depends mainly upon the ADA and SAGA histone acetyltransferase (HAT) complexes for which Gcn5 is the catalytic subunit. Previous screens have determined that global acetylation is reduced in cells lacking subunits of the Ccr4-Not complex, a global regulator of eukaryotic gene expression. In this study we have characterized the functional connection between the Ccr4-Not complex and SAGA. We show that SAGA mRNAs encoding a core set of SAGA subunits are tethered together for co-translational assembly of the encoded proteins. The Ccr4-Not complex is present at this site to promote the co-translational assembly of these subunits and this is needed for integrity of SAGA. In addition, we determine that a glycolytic enzyme, the glyceraldehyde-3-phosphate dehydrogenase Tdh3, a prototypical moonlighting protein, is tethered at this site of Ccr4-Not-dependent co-translational SAGA assembly and functions as a chaperone.
Keywords Ccr4-Not complexNot5Ada2Spt20Gcn5SAGATdh3Co-translational complex assembly
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URN: urn:nbn:ch:unige-865979
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Thesis (5.3 MB) - public document Free access
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Research group Groupe Collart Martine (microbiologie et médecine moléculaire) (81)
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KASSEM, Sari. Not5-dependent co-translational assembly of Ada2 and Spt20 is essential for functional integrity of SAGA. Université de Genève. Thèse, 2016. https://archive-ouverte.unige.ch/unige:86597

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Deposited on : 2016-09-05

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