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State transition7-dependent phosphorylation is modulated by changing environmental conditions, and its absence triggers remodeling of photosynthetic protein complexes

Bergner, Sonja Verena
Scholz, Martin
Trompelt, Kerstin
Barth, Johannes
Gäbelein, Philipp
Steinbeck, Janina
Xue, Huidan
Clowez, Sophie
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Published in Plant physiology. 2015, vol. 168, no. 2, p. 615-634
Abstract In plants and algae, the serine/threonine kinase STN7/STT7, orthologous protein kinases in Chlamydomonas reinhardtii and Arabidopsis (Arabidopsis thaliana), respectively, is an important regulator in acclimation to changing light environments. In this work, we assessed STT7-dependent protein phosphorylation under high light in C. reinhardtii, known to fully induce the expression of light-harvesting complex stress-related protein3 (LHCSR3) and a nonphotochemical quenching mechanism, in relationship to anoxia where the activity of cyclic electron flow is stimulated. Our quantitative proteomics data revealed numerous unique STT7 protein substrates and STT7-dependent protein phosphorylation variations that were reliant on the environmental condition. These results indicate that STT7-dependent phosphorylation is modulated by the environment and point to an intricate chloroplast phosphorylation network responding in a highly sensitive and dynamic manner to environmental cues and alterations in kinase function. Functionally, the absence of the STT7 kinase triggered changes in protein expression and photoinhibition of photosystem I (PSI) and resulted in the remodeling of photosynthetic complexes. This remodeling initiated a pronounced association of LHCSR3 with PSI-light harvesting complex I (LHCI)-ferredoxin-NADPH oxidoreductase supercomplexes. Lack of STT7 kinase strongly diminished PSII-LHCII supercomplexes, while PSII core complex phosphorylation and accumulation were significantly enhanced. In conclusion, our study provides strong evidence that the regulation of protein phosphorylation is critical for driving successful acclimation to high light and anoxic growth environments and gives new insights into acclimation strategies to these environmental conditions.
Keywords Chlamydomonas reinhardtii/metabolismEnvironmentMass SpectrometryMultiprotein Complexes/metabolismMutationPhosphorylationPhotosynthesisPhotosystem I Protein Complex/metabolismPlant Proteins/metabolismProteomics
PMID: 25858915
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Research groups Institute of Plant Biology and Biotechnology, University of Münster; Institut de Biologie Physico-Chimique, UMR 7141 CNRS-UPMC
Groupe Goldschmidt-Clermont
Swiss National Science Foundation: 31003A_146300
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BERGNER, Sonja Verena et al. State transition7-dependent phosphorylation is modulated by changing environmental conditions, and its absence triggers remodeling of photosynthetic protein complexes. In: Plant physiology, 2015, vol. 168, n° 2, p. 615-634. doi: 10.1104/pp.15.00072 https://archive-ouverte.unige.ch/unige:86430

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Deposited on : 2016-08-29

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