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Scientific article
English

Tumor promoter PMA stimulates the synthesis and secretion of mouse pro-urokinase in MSV-transformed 3T3 cells: this is mediated by an increase in urokinase mRNA content

Contributors

Belin, Dominique; Godeau, F.; Vassalli, Jean-Dominique

Published in

EMBO journal, vol. 3, no. 8, p. 1901-1906

Publication date

1984

Abstract

In mouse MSV-3T3 cells the synthesis of the urokinase form of plasminogen activator was increased 10-fold after addition of the tumor promoter phorbol-12-myristate-13-acetate (PMA). PMA also stimulated the secretion of the protein into the culture medium, mostly in the form of enzymatically inactive pro-urokinase. When assayed by injecting RNA into Xenopus laevis oocytes, the concentration of functional urokinase mRNA was found to be 6- to 10-fold higher in the PMA-treated cells; a similar increase in urokinase mRNA content was measured by hybridisation with a mouse urokinase cDNA probe. Thus, the induction of plasminogen activator by PMA in MSV-3T3 cells is accounted for by an increased content of urokinase mRNA.

Keywords

  • Animals
  • Carcinogens/ pharmacology
  • Cell Line
  • Cell Transformation, Viral
  • Enzyme Precursors/biosynthesis
  • Female
  • Fibroblasts/metabolism
  • Gene Expression Regulation/ drug effects
  • Mice
  • Oocytes/metabolism
  • Phorbols/ pharmacology
  • RNA, Messenger/biosynthesis
  • Tetradecanoylphorbol Acetate/ pharmacology
  • Urokinase-Type Plasminogen Activator/ biosynthesis/genetics/secretion
  • Xenopus laevis

UNIGE affiliation entities

Citation (ISO format)

BELIN, Dominique, GODEAU, F., VASSALLI, Jean-Dominique. Tumor promoter PMA stimulates the synthesis and secretion of mouse pro-urokinase in MSV-transformed 3T3 cells: this is mediated by an increase in urokinase mRNA content. In: EMBO journal, 1984, vol. 3, n° 8, p. 1901–1906.

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Identifiers

Additional URL for this publication

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC557615/pdf/emboj00312-0226.pdf

Journal ISSN

0261-4189
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Technical informations

Creation

12/07/2010 11:57:02

First validation

12/07/2010 11:57:02

Update

14/03/2023 15:50:08

Status update

14/03/2023 15:50:08

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