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Title

Tumor promoter PMA stimulates the synthesis and secretion of mouse pro-urokinase in MSV-transformed 3T3 cells: this is mediated by an increase in urokinase mRNA content

Authors
Godeau, F.
Published in EMBO Journal. 1984, vol. 3, no. 8, p. 1901-1906
Abstract In mouse MSV-3T3 cells the synthesis of the urokinase form of plasminogen activator was increased 10-fold after addition of the tumor promoter phorbol-12-myristate-13-acetate (PMA). PMA also stimulated the secretion of the protein into the culture medium, mostly in the form of enzymatically inactive pro-urokinase. When assayed by injecting RNA into Xenopus laevis oocytes, the concentration of functional urokinase mRNA was found to be 6- to 10-fold higher in the PMA-treated cells; a similar increase in urokinase mRNA content was measured by hybridisation with a mouse urokinase cDNA probe. Thus, the induction of plasminogen activator by PMA in MSV-3T3 cells is accounted for by an increased content of urokinase mRNA.
Keywords AnimalsCarcinogens/ pharmacologyCell LineCell Transformation, ViralEnzyme Precursors/biosynthesisFemaleFibroblasts/metabolismGene Expression Regulation/ drug effectsMiceOocytes/metabolismPhorbols/ pharmacologyRNA, Messenger/biosynthesisTetradecanoylphorbol Acetate/ pharmacologyUrokinase-Type Plasminogen Activator/ biosynthesis/genetics/secretionXenopus laevis
Identifiers
PMID: 6541126
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BELIN, Dominique, GODEAU, F., VASSALLI, Jean-Dominique. Tumor promoter PMA stimulates the synthesis and secretion of mouse pro-urokinase in MSV-transformed 3T3 cells: this is mediated by an increase in urokinase mRNA content. In: EMBO Journal, 1984, vol. 3, n° 8, p. 1901-1906. https://archive-ouverte.unige.ch/unige:8634

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Deposited on : 2010-07-12

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