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Scientific article
English

Clinical uses of anti-Müllerian hormone assays: pitfalls and promises

Contributors

Streuli, Isabelle; Fraisse, Timothee; Chapron, Charles; Bijaoui, Gérard; Bischof, Paul; De Ziegler, Dominique

Published in

Fertility and sterility, vol. 91, no. 1, p. 226-230

Publication date

2009

Abstract

OBJECTIVE: To investigate whether the controversy about fluctuations of anti-Müllerian hormone (AMH) levels during the menstrual cycle results from differences between the immunoassays currently available: the Beckman Coulter Immunotech kit (Fullerton, CA) and the Diagnostic Systems Laboratories kit (Webster, TX). DESIGN: Prospective trial. SETTING: Fertility clinics of two tertiary university hospitals. PATIENT(S): One hundred sixty-eight blood samples from three different populations. Serial samples at set intervals from the LH surge were taken in a fourth population of 10 volunteers. INTERVENTION(S): We remeasured AMH levels by using the Diagnostic Systems Laboratories kit in 168 blood samples in which AMH initially had been measured by using the Beckman Coulter assay. We also conducted serial AMH measurements (n = 7) during the menstrual cycle of 10 women. MAIN OUTCOME MEASURE(S): Linear regression of AMH levels determined by using 2 different assays and analysis of variance of serial measurements in the menstrual cycle. RESULT(S): We found a linear relationship between the 2 methods, with a correlation coefficient of 0.88. When repeated individual AMH measures were longitudinally analyzed in relation to the LH surge, a slight but significant decrease was observed after ovulation. CONCLUSION(S): Differences in AMH fluctuations during the menstrual cycle reported in recent publications do not result from the use of different AMH assays. The changes in AMH levels after ovulation are slight, yet statistically significant. However, the fluctuations observed are smaller than intercycle variability and therefore are not clinically relevant as far as AMH measurements for clinical purposes are concerned. In daily practice, AMH therefore can be measured anytime during the menstrual cycle.

Keywords

  • Adult
  • Anti-Mullerian Hormone/blood
  • Female
  • Humans
  • Immunoassay
  • Longitudinal Studies
  • Menstrual Cycle/physiology
  • Ovulation/physiology
  • Prospective Studies
  • Reference Values
  • Regression Analysis
  • Reproducibility of Results

UNIGE affiliation entities

Citation (ISO format)

STREULI, Isabelle et al. Clinical uses of anti-Müllerian hormone assays: pitfalls and promises. In: Fertility and sterility, 2009, vol. 91, n° 1, p. 226–230. doi: 10.1016/j.fertnstert.2007.10.067

Main files (1)

Article (Accepted version)

Identifiers

Journal ISSN

0015-0282
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Technical informations

Creation

19/02/2009 11:24:00

First validation

19/02/2009 11:24:00

Update

14/03/2023 15:00:46

Status update

14/03/2023 15:00:46

Last indexation

29/10/2024 11:10:45
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