Scientific article

Evaluation of a new low sheath–flow interface for CE-MS

Published inElectrophoresis, vol. 37, p. 936-946
Publication date2016

The current trend for increasing technical complexity in the field of CE-ESI-MS interfaces has incited for more accessible alternatives. In this work, a simple low sheath-flow ESI interface operating in the submicroliter nanospray regime without nebulizing gas assistance was evaluated. The use of sheath liquid enabled improving the ionization of the analytes, while the absence of nebulizing gas minimized sample dilution and loss of efficiency. After a rapid qualification, the effect of main operational parameters such as sheath liquid composition and flow rate, working distance and ESI potential was studied. Simulation of the mixing processes inside the Taylor cone proved its size to be of utmost importance in band broadening processes. As a proof of concept, the interface was eventually applied to a set of representative basic drugs analyzed by CE-TOF/MS. Limits of detection reached the 25–100 ppb range with suitable robustness and repeatability results. This design has demonstrated good performance while being simple and accessible to the user.

  • CE-MS interface
  • Electrospray
  • Low sheath-flow
Citation (ISO format)
GONZALEZ RUIZ, Victor et al. Evaluation of a new low sheath–flow interface for CE-MS. In: Electrophoresis, 2016, vol. 37, p. 936–946. doi: 10.1002/elps.201500523
Main files (1)
Article (Published version)
ISSN of the journal0173-0835

Technical informations

Creation08/10/2016 12:07:00 PM
First validation08/10/2016 12:07:00 PM
Update time03/15/2023 12:36:59 AM
Status update03/15/2023 12:36:58 AM
Last indexation08/30/2023 9:00:28 AM
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