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Title

Isolation and initial characterization of a novel zinc finger gene, DNMT3L, on 21q22.3, related to the cytosine-5-methyltransferase 3 gene family

Authors
Aapola, Ulla
Kawasaki, K.
Ollila, J.
Vihinen, M.
Heino, M.
Shintani, A.
Minoshima, S.
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Published in Genomics. 2000, vol. 65, no. 3, p. 293-298
Abstract We have isolated the DNMT3L gene that is related to the cytosine-5-methyltransferase 3 (DNMT3) family. The gene is located on chromosome 21q22.3 between the AIRE and the KIAA0653 genes and spans approximately 16 kb of genomic sequence. The encoded protein of 387 amino acids has a cysteine-rich region containing a novel-type zinc finger domain that is conserved in DNMT3A and DNMT3B but also in ATRX, a member of the SNF2 protein family. The novel domain, called an ADD (ATRX, DNMT3, DNMT3L)-type zinc finger, contains two subparts: a C2C2 and an imperfect PHD zinc finger. Expression of the DNMT3L mRNA was not detectable by Northern blotting; however, RT-PCR amplification revealed that it is expressed at low levels in several tissues including testis, ovary, and thymus.
Keywords Amino Acid SequenceAnimalsChromosome MappingChromosomes, Human, Pair 21/ geneticsDNA (Cytosine-5-)-Methyltransferase/chemistry/ genetics/metabolismHumansMiceModels, MolecularMolecular Sequence DataProtein ConformationProtein Structure, TertiarySequence AlignmentZinc Fingers/ genetics
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PMID: 10857753
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AAPOLA, Ulla et al. Isolation and initial characterization of a novel zinc finger gene, DNMT3L, on 21q22.3, related to the cytosine-5-methyltransferase 3 gene family. In: Genomics, 2000, vol. 65, n° 3, p. 293-298. https://archive-ouverte.unige.ch/unige:8593

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Deposited on : 2010-07-12

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