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COP1 is required for UV-B-induced nuclear accumulation of the UVR8 photoreceptor

Published in Proceedings of the National Academy of Sciences. 2016, vol. 113, no. 30, p. E4415-22
Abstract The UV-B photoreceptor UV RESISTANCE LOCUS 8 (UVR8) promotes UV-B acclimation and tolerance in Arabidopsis thaliana UVR8 localizes to both cytosol and nucleus, but its main activity is assumed to be nuclear. UV-B photoreception stimulates nuclear accumulation of UVR8 in a presently unknown manner. Here, we show that CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1) is required for UV-B-induced nuclear accumulation of UVR8, but bypassing the COP1 requirement for UVR8 nuclear accumulation did not rescue the cop1 mutant UV-B phenotype. Using a glucocorticoid receptor (GR)-based fusion protein system to conditionally localize GR-UVR8 to the nucleus, we have demonstrated that both photoactivation and nuclear localization of UVR8 are required for UV-B-induced photomorphogenic responses. In contrast, there was no UV-B response when UV-B-activated UVR8 was artificially retained in the cytosol. In agreement with a predominantly nuclear activity, constitutively active UVR8(W285A) accumulated in the nucleus also in the absence of UV-B. Furthermore, GR-COP1 expression lines suggested that UV-B-activated UVR8 can be coimported into the nucleus by COP1. Our data strongly support localization of UVR8 signaling in the nucleus and a dual role for COP1 in the regulation of UV-B-induced UVR8 nuclear accumulation and in UVR8-mediated UV-B signaling.
PMID: 27407149
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Other version: http://www.pnas.org/content/113/30/E4415.full
Research group Groupe Ulm
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YIN, Ruohe et al. COP1 is required for UV-B-induced nuclear accumulation of the UVR8 photoreceptor. In: Proceedings of the National Academy of Sciences, 2016, vol. 113, n° 30, p. E4415-22. doi: 10.1073/pnas.1607074113 https://archive-ouverte.unige.ch/unige:85615

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Deposited on : 2016-08-02

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