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Peptide degradation during preparation and in vitro release testing of poly(L-lactic acid) and poly(DL-lactic-co-glycolic acid) microparticles

Contributeurs/tricesWitschi, Claudia; Doelker, Eric
Publié dansInternational journal of pharmaceutics, vol. 171, no. 1, p. 1-18
Date de publication1998
Résumé

Biodegradable, tetracosactide-loaded microparticles were prepared by means of (i) spray drying, (ii) w/o/w solvent evaporation method (WOW) and (iii) by the aerosol solvent extraction system (ASES) using poly(l-lactic acid) (l-PLA) and poly(dl-lactic-co-glycolic acid) (dl-PLGA) of varying monomer composition or molecular weight. In the absence of the polymer the peptide did not degrade or aggregate irreversibly when in contact with methanol and methylene chloride or under the conditions used in the first step of WOW, as proven by HPLC, electrospray-mass spectrometry (MS) and circular dichroism (CD). During the extraction process, used to isolate the peptide from the microparticles, tetracosactide was partially oxidised. The highest stability of the peptide during microencapsulation was guaranteed with high molecular weight l-PLA, when using WOW or ASES, and with very low molecular weight PLGA, in the case of spray drying and WOW. The burst release of the microparticles, during in vitro release testing, depended on the preparation method as well as on the nature of the polymer and increased in the order ASES

Mots-clés
  • Microparticles
  • Microspheres
  • Preparation methods
  • Poly(L-lactic acid)
  • Poly(DL-lactic-co-glycolic acid)
  • Peptide degradation
  • Peptide adsorption
  • Tetracosactide
  • In vitro release
Citation (format ISO)
WITSCHI, Claudia, DOELKER, Eric. Peptide degradation during preparation and in vitro release testing of poly(L-lactic acid) and poly(DL-lactic-co-glycolic acid) microparticles. In: International journal of pharmaceutics, 1998, vol. 171, n° 1, p. 1–18. doi: 10.1016/S0378-5173(98)00155-0
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Article (Published version)
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Identifiants
ISSN du journal0378-5173
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Création06.07.2016 16:04:00
Première validation06.07.2016 16:04:00
Heure de mise à jour15.03.2023 00:34:13
Changement de statut15.03.2023 00:34:13
Dernière indexation16.01.2024 21:20:55
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