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Title

Autoimmune T lymphocytes in myasthenia gravis : determination of target epitopes using T lines and recombinant products of the mouse nicotinic acetylcholine receptor gene

Authors
Melms, A.
Chrestel, S.
Schalke, B.C.
Wekerle, H.
Ballivet, M.
Barkas, T.
Published in Journal of Clinical Investigation. 1989, vol. 83, no. 3, p. 785-790
Abstract Oligoclonal and cloned T lines from peripheral blood or thymuses of patients with myasthenia gravis (MG) were selected for reactivity against nicotinic acetylcholine receptors (AChR) from Torpedo california, or against a recombinant fusion peptide, X4, representing the extracellular portion of the mouse AChR alpha-chain. All cell lines expressed the CD4 membrane phenotype, and their antigen reactivity was blocked by antibodies against monomorphic HLA DR/DP determinants. Using a panel of fusion proteins of different, overlapping mouse AChR alpha-chain sequences, a major T cell epitope was localized between amino acid positions 85 and 142. This determinant was distinct from the humoral main immunogenic region, which has been identified on the sequence 61-76. The response pattern of uncloned T lines from three patients with different HLA haplotypes suggests, however, that in any one MG patient T lymphocytes may recognize more than one autoantigenic epitope on the AChR alpha-chain, and that the T lymphocyte response profiles vary among individual patients.
Keywords AdultAmino acid sequenceAnimalsAntigens, differentiation, T-lymphocyte analysisAutoantigens immunologyAutoimmune diseases immunologyCells, culturedEpitopes immunologyFemaleHLA-DR antigens genetics immunologyHaplotypesHumansMaleMiceMyasthenia gravis immunologyReceptors, nicotinic genetics immunologyRecombinant proteins immunologyT-lymphocytes immunologyTorpedo
Identifiers
PMID: 2466050
Note PMCID: PMC303748
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MELMS, A. et al. Autoimmune T lymphocytes in myasthenia gravis : determination of target epitopes using T lines and recombinant products of the mouse nicotinic acetylcholine receptor gene. In: Journal of Clinical Investigation, 1989, vol. 83, n° 3, p. 785-790. https://archive-ouverte.unige.ch/unige:85389

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Deposited on : 2016-07-20

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