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Autoimmune T lymphocytes in myasthenia gravis : determination of target epitopes using T lines and recombinant products of the mouse nicotinic acetylcholine receptor gene

Published inThe Journal of clinical investigation, vol. 83, no. 3, p. 785-790
Publication date1989
Abstract

Oligoclonal and cloned T lines from peripheral blood or thymuses of patients with myasthenia gravis (MG) were selected for reactivity against nicotinic acetylcholine receptors (AChR) from Torpedo california, or against a recombinant fusion peptide, X4, representing the extracellular portion of the mouse AChR alpha-chain. All cell lines expressed the CD4 membrane phenotype, and their antigen reactivity was blocked by antibodies against monomorphic HLA DR/DP determinants. Using a panel of fusion proteins of different, overlapping mouse AChR alpha-chain sequences, a major T cell epitope was localized between amino acid positions 85 and 142. This determinant was distinct from the humoral main immunogenic region, which has been identified on the sequence 61-76. The response pattern of uncloned T lines from three patients with different HLA haplotypes suggests, however, that in any one MG patient T lymphocytes may recognize more than one autoantigenic epitope on the AChR alpha-chain, and that the T lymphocyte response profiles vary among individual patients.

Keywords
  • Adult
  • Amino acid sequence
  • Animals
  • Antigens, differentiation, T-lymphocyte analysis
  • Autoantigens immunology
  • Autoimmune diseases immunology
  • Cells, cultured
  • Epitopes immunology
  • Female
  • HLA-DR antigens genetics immunology
  • Haplotypes
  • Humans
  • Male
  • Mice
  • Myasthenia gravis immunology
  • Receptors, nicotinic genetics immunology
  • Recombinant proteins immunology
  • T-lymphocytes immunology
  • Torpedo
NotePMCID: PMC303748
Affiliation Not a UNIGE publication
Citation (ISO format)
MELMS, A. et al. Autoimmune T lymphocytes in myasthenia gravis : determination of target epitopes using T lines and recombinant products of the mouse nicotinic acetylcholine receptor gene. In: The Journal of clinical investigation, 1989, vol. 83, n° 3, p. 785–790. doi: 10.1172/JCI113958
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Article (Published version)
accessLevelPublic
Identifiers
ISSN of the journal0021-9738
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